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Summary Attributions
XB-CLONE-3336071

Clone Name: IMAGE:7763044 Gene: acta1 Species: Xenopus tropicalis  
 

Sequence:

5' EST: DR888082 [+]

3' EST: DR888081 [+]

Library:

Name: NIH_XGC_tropMet2External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pSPORT1 vector. This library contains the 1.5-2 kb size fraction (the 0.5-1.5 kb fraction is contained in the NIH_XGC_tropMet3 library). Library constructed at the DOE Joint Genome Institute.

Anatomy: whole organismStage: NF stage 62
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pSPORT1

Type: phagemid

5' Restriction Site: SalI

3' Restriction Site: NotI		Expression Image

Usage:

Suppliers:

Search for clone at: Open Biosystems