XB-CLONE-3337768
Clone Name: IMAGE:7796437
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR886994
[+]
cggtcggattccgggtcgacagcgcttcgcccatctggttgaactgcgggccaactggccgatttcgtcatggctggaaacattcaggcgcgcgctcaaa
tcaccggcgcccagggcttctgcctgacgtaccaggtcgcccaaaggctgcaacttgctgcgcagcagccagaccgccgcgcccaccgccagcagcatcg
cgagcaaactgccgatcgccaggcgaatccccaccgaccaggtcaccgcgcgaatttcggctttcggcatgctcgccaccaccgaccacgggccgccttc
aaacggcaccgcgacgctgtagaaatcttcgttcttgtcgctccagaaacggccttttccaggtttggcggccaggtcgagcatgaccggaatcgactga
tccagcgcctgcaccccagctggcgggaccaaccagtgtttctgttcgtccagcagcgccagggaaccggtctggccgatgcggaagcgcttgaggttgt
cgaactgggcgttctgcgcgtcggtgtagtcgaagccgacaaacagcaccgcaatcactttgccgctggcatcgcgcaccgggctgtactgggtcatgta
ggagcgatcaaacaacaccgcgcggccgacgtagccctgcccgccgagcaagcgttgataggccgggtgctgatgatccagcgccgtgccaatggcgcgg
ctgccgtcctgcttggtcagggatgtgctgacgcggataaagtcctcgccgctgcgtacgaatacggtcgccacgccgccggacatctgcttgaactcat
caacttcagcgaaattgttgttgagcacttcctcgcccaggtacaggcccggcgtctgcacgcccgccac
3' EST: DR886993
[+]accttgcaactcaccgaggcggcgcgcctgttgctgccgggcgtgcgcgaaggctttgcggccctggagcgcgcctgccatacattgaatgcggaggacg
acatcctgcgcatgaaggcgccgtccaccctgaccatgcgttggctgctggcacggctcagtcgcttccggcatttgcagccgggcaatgaagtgcaact
gaccagcgcctggatgagtgtcgatgaggtggacttcaaccaggagccgttcgattgcgcggtattgctgagtaacggtcatttcccgccggactgggaa
gcctgctacctgtttcctgaactactgattccagtgggcgcgccgaacctgttgagcgacgggccctgggacgtcacgcggctggcctctgccgaattgc
tgcacccgacgccggaccggcgtgactggcgctattggctggagcgcatggggctgtcatcccaggtatcgctcaagggcgggcaggtgttcgacacatt
ggagctgggcatgattgccgccgcgcgggggtacggcgtgtccatgggtgacttgctgatggtggctgaagacgtggcccagggccgcctgagcctgcca
tggcccaccgcggtggccagtggggaaaattactatctggtgtggccaaagacccggcccggcggtgagcgtttgcgacggctgggtgactttctccaga
gcgaggtgcaggccatggaattgccccaggtagagcgcctcgcctgagcggattgcgcaccgggcagcaatcgtttgcgtgatacccctgcgaatcgctc
aagtattgagcctgctcg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pSPORT1 vector. Library constructed at the DOE Joint Genome Institute.Vector Details | Vector Map |
Name: pSPORT1
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40561.jpg) |
Usage:
Suppliers: