XB-CLONE-3358273
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Clone Name: IMAGE:7842894
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Gene:
eif4e2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121524 
[+]
GCGATTTTGGACTGGAAGTCCCGGGAAGGGCTGCAGCGTTGGGCGGTATGGACTTGATAGGAAATGGGTTTGTCTGGACAGGAAGACCTCACAACCGCAG
AAGATGACTTCACCAAATCTCAGAAGGTCAAGGAGGTGATGGTGCCCCCGGGGGAACACCCGCTCCAGTACAAATACACCTTCTGGTACTCTAGGAGAAC
CCCATCCAGACCTGCAAGTACCCACAACTATGAACAGAATATCCGCCAGTTTGGCACCGTAGCGTCGGTGGAGCAGTTCTGGAGAATCTACAGCCACATA
GTACGTCCTGGTGATCTGACAGGCTACAGTGACTTCCATCTCTTTAAGGACGGCATCAAACCCATGTGGGAGGACGAAGCCAATAAAAATGGCGGGAAGT
GGATCATTCGCCTTCGGAAAGGCCTGGCTTCCAGATTCTGGGAGAACATCATCTTGGCCATGTTAGGGGAGCAGTTTATGGTCGGGGAAGAGATCTGCGG
GGTGGTCGTATCAATCCGCTTCCAGGAGGATATCCTGTCTATCTGGAACAAGACGGCCAATGACCAGTTCAGCACCGTGAGAATCCGGGACACACTAAGG
AGAGTGTTAAATCTGCCCCCCAATACCATAATGGAATACAAAACCCACACTGACAGCCTTAAGGATAACTCCAGCTTCCGTAACACAAAGATCACGGTAT
AGCACTGGGCAGGTCCGCCATAGCCGCGGCCAAAATGGGAAACTGTCTGTGAACTGCTGCGTGACTGGGTTGTTGGTTTTTGTTACTTCCGTGCGTACAG
AAGCAATCGTAGCCCCTCCCCCTGCGCTGCCTCTGTCCGTTATGTCATAGAAACTCAATAAATTATACCTATTTTTTCAAAAAAAAAAAAAAAAAA
5' EST: DR871471 [+]cgattttggactggaagtcccgggaagggctgcagcgttgggcggtatggacttgataggaaatgggtttgtctggacaggaagacctcacaaccgcaga
agatgacttcaccaaatctcagaaggtcaaggaggtgatggtgcccccgggggaacacccgctccagtacaaatacaccttctggtactctaggagaacc
ccatccagacctgcaagtacccacaactatgaacagaatatccgccagtttggcaccgtagcgtcggtggagcagttctggagaatctacagccacatag
tacgtcctggtgatctgacaggctacagtgacttccatctctttaaggacggcatcaaacccatgtgggaggacgaagccaataaaaatggcgggaagtg
gatcattcgccttcggaaaggcctggcttccagattctgggagaacatcatcttggccatgttaggggagcagtttatggtcggggaagagatctgcggg
gtggtcgtatcaatccgcttccaggaggatatcctgtctatctggaacaagacggccaatgaccagttcagcaccgtgagaatccgggacacactaagga
gagtgttaaatctgccccccaataccataatggaatacaaaacccacactgacagccttaaggataactccagcttccgtaacacaaagatcacggtata
gcactgggcaggtccgccatagccgcggccaaaatgggaaactgtctgtgaactgctgcgtgactgggttgttggtttttgttacttccgtgcgtacaga
agcaatcgtagccc
3' EST: DR871470 [+]gaaaaaataggtataatttattgagtttctatgacataacggacagaggcagcgcagggggaggggctacgattgcttctgtacgcacggaagtaacaaa
aaccaacaacccagtcacgcagcagttcacagacagtttcccattttggccgcggctatggcggacctgcccagtgctataccgtgatctttgtgttacg
gaagctggagttatccttaaggctgtcagtgtgggttttgtattccattatggtattggggggcagatttaacactctccttagtgtgtcccggattctc
acggtgctgaactggtcattggccgtcttgttccagatagacaggatatcctcctggaagcggattgatacgaccaccccgcagatctcttccccgacca
taaactgctcccctaacatggccaagatgatgttctcccagaatctggaagccaggcctttccgaaggcgaatgatccacttcccgccatttttattggc
ttcgtcctcccacatgggtttgatgccgtccttaaagagatggaagtcactgtagcctgtcagatcaccaggacgtactatgtggctgtagattctccag
aactgctccaccgacgctacggtgccaaactggcggatattctgttcatagttgtgggtacttgcaggtctggatggggttctcctagagtaccagaagg
tgtatttgtactggagcgggtgttcccccgggggcaccatcacctccttgaccttctgagatttggtgaagtcatcttctgcggttgtgaggtcttcctg
tccagacaaacccattttctatcaagtccataccgcccaacgctgcagccctt
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 978 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: stomach | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
