XB-CLONE-3372399
|
Clone Name: IMAGE:7832033
|
Gene:
dcaf6
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
Full length sequence: BC121610 
[+]
GATACATGGCTGTCAGAGCGTCCTTTACCGCTTCCGGCCCTGTGTATTGCTCGCACTGAGCGCCTGACAGAACTTTACAGCGGGAGCCTGTAACAGGCAG
AGTGGCCGCCTTCCTTTAAGCTGTAGTCGTGCACAGTTTTTCCCGATGAAGTCAAGCATCTGCGCTTTTGTTTAGTCACGAGTCGCTATTACCTTGCGCT
AAGAAATCCGATTGCCATTTTGGACAAGCCAGCCTGAAAGGATATACAGGAACTGGACTATGTCACGGTGCTATTCTGGTCTAATATGGGATGTACGGAA
ACGCTCTCTTGGATTGAGTGAGCCCGATGTGGTCCGTAGCAACTATTTGGGAAGAAGAGAGTTTGTGCAAAGACTTAAATTGGAAGCAACATTAAACATT
CATGATGGTTGTGTAAACTCAATTTCTTGGGGTAGTACAGGAGAATACATTTTATCTGGTTCTGATGATACCACTTTGGTTATCACTAACCCGTACAATA
AAAAAGTCCTGACAAGAATTCGATCAGGTCACAGAGCCAATATTTTTAGTGCAAAGTTCCTGCCTCTTACCAATGACAAGCAGATTATATCCTGCTCCGG
AGATGGAGTTATATTTTTTACAGATATTGAGAAAGATGCAGACACAAACAGGCAATGTCAATTTACATGTCATTATGGAACCGCATATGAGATCATGACA
GTACCCAATGATCCCTACACATTTTTGTCCTGTGGTGAAGATGGAACGGTTAGATGGTTTGATACCAGGACGAAGACCAGCTGTACTAAAGAAGACTGCA
AAGATGATATTCTAATAAACTGCAGACGAGCTGCTACCTCAATTGCAATTTGTCCAACGGCACCATACTATCTTGCAGTTGGATGTTCGGACAGCTCAGT
GAGAATATATGATCGCCGTATGCTTGGAACTAGAGCAACGAACAATTATTCAAATCGTGGAACTACAGGAATGTGTGTCCGCTTTGTTCCCTCACATCTT
ACCAACAAATCCTGCAGAGTGACCTCTTTATGCTATAGTGAAGATGGGCAAGAGGTTCTAGTGAGCTACTCCTCTGACTACATTTACCTATTTGATCCTA
AAAATGACCAAGCAAAAGAGCTCAAATTGCCATCATCTGATCAGAAAAGGGAGGAGGTAAGGCAACCCCCTGTTAAGCGCCTAAGACTCCGAGGAGATTG
GTCTGATACAGGTCCAAGGGCAAGACCTGAAAGTGAACGAGAAAGAGATGGAGATCAAAGCACCAATGCATCACTAATGCAAAGAATGTCTGACATGCTC
TCAAGGTGGTTTGAAGAGGCTAGTGAAGTTGCACAGAGCAGCAGAAGACGAAACAGACTGCAAAATAGAGCAGGAACTTCACAGCCTATTTCATCAGCTT
CACTGTCTACAAGCCATACAGAGACCCAGGCATCAGAAACTTTTCCAGAGGTGGCTGCCTCCTCAGAACAGCCATCTTCCTCATCAAATGTTGAAGAAAG
TCACTCAATGACGTCTATAGCTACTGAACAACTAGAAACTTTTGCTTCATCAGATGGAGGCTCCAACCCTGCTGAGAGAGCTAAAGTAACCTCAGAGAGA
GAGAAAAATGAGCCGGTACTGCAATTACATTATAGCACTGAAGGAACAACAACAAGTACAATAAAGCTGGACTTCACAGATGAATGGAGCAATAGATCTT
CAAGTTCTGTAGCTGTGAAAGTCGGGAACAATGAAAGTACTGCAACTACTGAACTGACACAACCAAGTGAAATGGAAGACACATCTGAAAGTACAGAGCA
GCATGTAGAGACAACATGCAGTGACCCTGGAATGGAACAAGAAAGCCCTGGAAAATCCAACAGAAGCTCAAATAATGAAGGACTTCCAGATACATCTGTG
ACTGCAGCAACATCTGTAAGCTCTACGCACCAGGAGACTGACAGTGATGATGACACTGTTCGAGTCCCATCCAGAACCCAATCACAAAATAGGAGAACTG
CAGCAGCTCGTATCCAGGAGCTCTTTAGAAGAAGAAAAGAAAGAAAAGAGCTAGAAGAGATGGACATGCAAAATGTACATCAGCCCTCCTCAAAAATAGT
GTACAAAGGTCACCGGAATTCACGAACAATGATTAAAGAGGCTGCATTTTGGGGCAAAAACTTTGTCATGAGTGGATCTGATTGTGGTCACATCTTCATC
TGGGATCGGCATACAGCAAATCATTTAATGCTTTTAGAAGCTGATAATCATGTGGTAAACTGTCTGCAGCCACACCCATATGACCCAATTTTAGCATCAT
CTGGAATAGACTACAATATTAAGATATGGTCTCCTCTTGAACAAGACAAATGCTTTAACTGGAGACTTGCTGAAGAGGTGATTTCACGGAATGAGTTAAT
GCTGGAAGAAACAAGAAACACTATTACAGTCCCAGCATCTTTCATGCTAAGAATGTTGGCTTCACTCAACCATATACGGGCAGATCGAGGAGAAGACCGA
CCTGAAGGAGCCGGACAGGATAACTCCAATGAAGATGAATGATTGTACTTCTCTTATGAGGATTTAAGCATATTGCATTATGTAAAAGCAATTTATTATC
ATTTTCCTAAATGGATTTGGCAAATTTTATTTTATAATATGAAAGTTTTGTATTGTGACTGAATGAAGAGCCTACAAAATATTGCCAGCTTCTACTCAAG
ATTGAATATATATATATATCTATATATATATCCTGATAGGAAAAATTGTTTGGGTGTGACATGTAAATAAACTCTGGTAACTTATTGTTTTAAATTTAAA
AAAAAAAAAAAAAAA
5' EST: DR851555 [+]ccgagggatacatggctgtcagagcgtcctttaccgcttccggccctgtgtattgctcgcactgagcgcctgacagaactttacagcgggagcctgtaac
aggcagagtggccgccttcctttaagctgtagtcgtgcacagtttttcccgatgaagtcaagcatctgcgcttttgtttagtcacgagtcgctattacct
tgcgctaagaaatccgattgccattttggacaagccagcctgaaaggatatacaggaactggactatgtcacggtgctattctggtctaatatgggatgt
acggaaacgctctcttggattgagtgagcccgatgtggtccgtagcaactatttgggaagaagagagtttgtgcaaagacttaaattggaagcaacatta
aacattcatgatggttgtgtaaactcaatttcttggggtagtacaggagaatacattttatctggttctgatgataccactttggttatcactaacccgt
acaataaaaaagtcctgacaagaattcgatcaggtcacagagccaatatttttagtgcaaagttcctgcctcttaccaatgacaagcagattatatcctg
ctccggagatggagttatattttttacagatattgagaaagatgcagacacaaacaggcaatgtcaatttacatgtcattatggaaccgcatatgagatc
atgacagtacccaatgatccctacacatttttgtcctgtggtgaagatggaacggttagatggtttgataccaggacgaagaccagctgtactaaagaag
actgcaaagatgatattctaataaactgcagacgagctgctacct
3' EST: DR851554 [+]aaatttaaaacaataagttaccagagtttatttacatgtcacacccaaacaatttttcctatcaggatatatatatagatatatatatatattcaatctt
gagtagaagctggcaatattttgtaggctcttcattcagtcacaatacaaaactttcatattataaaataaaatttgccaaatccatttaggaaaatgat
aataaattgcttttacataatgcaatatgcttaaatcctcataagagaagtacaatcattcatcttcattggagttatcctgtccggctccttcaggtcg
gtcttctcctcgatctgcccgtatatggttgagtgaagccaacattcttagcatgaaagatgctgggactgtaatagtgtttcttgtttcttccagcatt
aactcattccgtgaaatcacctcttcagcaagtctccagttaaagcatttgtcttgttcaagaggagaccatatcttaatattgtagtctattccagatg
atgctaaaattgggtcatatgggtgtggctgcagacagtttaccacatgattatcagcttctaaaagcattaaatgatttgctgtatgccgatcccagat
gaagatgtgaccacaatcagatccactcatgacaaagtttttgccccaaaatgcagcctctttaatcattgttcgtgaattccggtgacctttgtacact
atttttgaggagggctgatgtacattttgcatgtccatctcttcnagctcttttctttcttttcttcttcnaaagagctcctggatacgagctgctgcag
ttctcctattttgtgattgggttctggatgggactcgaacagtgtcatcatcactgtcag
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: ovary | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
