XB-CLONE-3390147
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Clone Name: IMAGE:7652069
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Gene:
elfn2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122901 
[+]
GTGCCTGTCTCTGGCGCCGCAAGGGACGCAGCTCTGCTGGTGACACCGTATTGGGGGGAACATTGGTGACAGGAAGAGCAAGGAAAGTGACAGGGACACC
GGTTGCTAAGCGGACCAGCAGATCCTTCACGCCAGAGAAGATCACGAGAGGAAGAGCCAAGTGATACAGACACTGATGCTGGTGGCAGCTGGAGACGGAC
AGACACAGAGACGGATTCATCTCTTGAAATTCACGTCCCGGGGGTACAGAGTCGGCTGGCACAGACACATATACTTTCTATCTTCTGTTCTGTCTCCCTT
CATCAATTCCATCTATGCTCTGTTTCCTTGATTTTATAGAGGAAGAAGAGAGACTACAATTAAAGTACTGTTGACAGCAAACAAGGTGCAGGCTAAGTGG
CCATATAACCTGTGTACGATTTTTGTGCAAGCATCCTTCCTTTCAAAAGATAATGTGCAAAATTTTGTTGAGGAATGTAATACACGGGCTTCGCTAAGTT
GTGGCACTGAGTTTTTCCCAACTGTACGCTTCATTTTTTCCAACTAAAAAATTATTTTTAGTACCACAATGTCATGCTGGTGGCTTTGGCTGACAACCGT
CTTTTTCTTTTGCCACCTGCCTACTGGTGCACGATCTGATTGCTGGCTGATTGAGGGGGAAAAAGGATATGTCTGGCTAGCAATCTGCAGCCAGAACCAA
CCCCCCTATGAGACCATACCTCAGCACATCAATAGCACAGTTCATGACCTTCGGCTCAATGAAAACAAGTTAAAGATAATTGGTTATTCCTCACTATCCC
GCTTTGGAAACTTGACAGATCTGAACCTGACCAAGAATGAGATCTACTACATAGAAGATGGAGCCTTCATGGGGCAGTCTAACCTACAGATCCTGTATCT
GGGCTATAATAAACTGACCAACCTATCTGAGGGAATGTTAAGGGGAATGCCCAGGCTGCAGTTTCTTTTTGTTCAGCATAACCTGATAGATGTGGTAACA
CCTAATGCCTTTTCTGAGTGCTTGAGCCTTATCAGTATCGATTTGTCCTCAAATAGAATAAAAAATCTAGATAGTTCAACATTTGCTGGTCTTCAGACTC
TTATGGTATGTGAATTGGCTGGGAACCCTTTCCATTGTGGATGTGAGCTCTATGGCTTTCTTGAATGGTTAGTCATGTTTAACAATGTCACACGCAACTT
TGATCGACTACAGTGTGAAACCCCACGTGAATTTACTGGGTTTCCACTGCTAAGCCCAAGACCACATCATAGCAGAAATGCTATAACTGTTCTTCAAGCT
CACTGTAGGAACGGAGGACACCTCCATCTGCCAAGGGAGAGGCCCACATCACTAAGTGCAGACTCCTTACATGAAACAGATGAGAGCTCTGGCTTCAATG
CTGGGGATATTCTGTCGCCAGAAACTACTACACCTACTCCAACCCCTGACTCTTCAGGTGGCCCCACAATAGACATTCACCATGTAACAGGAAACTCAGC
CACCCTGATTGTAACAATTCCTTATCCCTTTCAAAAACTCTATGTTTTAGTGCAATACAATAACAGCTTTGTATATGATGTGACAACTCTGAAGCACAAG
AAGGAATATATTACTCTGGACAAACTAAAACCTCATGTGAACTATACATTTTGTGTGACTTCCATACGCTTTTCGAAACGATACAACCACACGTGTTTAT
TCCTTGCCACTCGTATCAGAGATGAGAAAGACTTCGCTCCCAGTACTTCTACTACAACTCACTATATTATGACAATATTAGGTTGTCTTTTTGGCATGGT
CATCATCTTGGGAGTAGTTTACTATTGTTTAAGGAAGAAGAGGATGCAGGAAGAAAAGAAGAAATCTCTGAATGTTAAGAAAACCATTCTAGAAATGAGA
TATGGTTCAGATGTCGATCCCTGCTTAGGGCCTCATTCCTCACAGAAGTTATCTGAGCACCCCATTCCTATTTCTTGCATATCTTCACTCCCTACATCTG
TATCTGGGCTTGGGAGTGGAGGAGAGAAAGGAATGTCCTCCAAGCAAATAAATTCTCAAATGGGGACGCCAAAAGGCACAAACTACATGGAAGTTCGGAG
TGGAGATGGGTTAGACAGAGGCCAAAGGAGGATGTCAGGTGAAGAAGAGGATGATGATGACTTTCGTGAACTTGACAATGGTGAGGGATCTGCTTCTGAA
ATATCCACCATTGCCAAGGAGGTAGACAAAGTGAATCAGATCATTAACAACTGTATTGATGCCCTCAAGCTGGACACTGCCTCTTTTTTAGGCGGGGGGG
ACCCGGAGCTAGGCTACGACTGCCAGTCCGTGCCAGCCAGCTCATCGGGCCACTTGGAGAGGCTGAGCTTCCTCTCTCCTGCATACAAGGAGGGTGTGCA
CCCTCTTCAGCGCCAACTCAGTGCAGATGCAGCCACAGTGGGCAAGAAACGCTGCAGCATTTCATCTAGTGGCTCCATTAAAAGTGCCCGAGTTTTCAGT
TTGGATGTTCCTGACCAATCTTTAAAATGTGACTCCAAATATATTGAAAAAAGCAGTCCCCTTAACAGCCCTTTGGATCGCCTTCCTCTAGTGTCTTCTT
CTGGAGTACAACACCTAGACGTCAAGCCTTCCTACCACTGCAGTGAGCACCGGCATTCCTTCCCAGCCCTCTATTATGAGGAAAGCGCTGATACATTAAG
CCAGAGGGTCAGCTTCCTAAAACCCCTTTCCCGCTCTAAAAGAGACTCCAGCTACTCCCAACTGTCTCCTAGACACCATTTCTCTGGATACTCCTCAAGC
CCAGAGTACTCCACTGAAAATACCCACAAGATCTGGGAGCGCTTCCGTCCTTACAAAAAACACACCCGGGAAGAGGTCTATATAGCAGCTGGACATGCTC
TGCGCAAAAAAGTCCAGTTTGCTAAAGGGGAGGACTTGCATGATATTTTGGATTACTGGAAAGGGGTATCTGCACAACAAAAACTGTGACTGGCACCTTA
AAAGGTGTAAAAGGTATAAAAGGATGTCAGGGAAGTTGACAGTTTTTTGTTGAGAACTGTGGTGTTGACGTATCACAGTTTGCAATTAAAGTTTACATTG
GAGGTGGGGGGCTGTACCTCATAAAGGTGGGGAGGAGAAAGATGGACTTGCGGGTTGGAGAGGACATTTTCAGGCACAGTTCTCCCCCCTTTAAAACACC
ACCCCCTCCATTTCACACACTGTACTAGTGGCTTTCTCTCCATTAATATATTTAATTTAAGTTTAACTTTTATTCCCCCTCAAAGCTGTCGTAAGCTGAA
TTCACACAGCAATAAGGCACTTCAGGGTTTTAAATATGTTTGAAAACCTATTTTGTGTATTTATTTTGAAAAGCATGATGAAATTCAGTTCACAAATGGT
GCAAAATAATAGGGTTACCTTAAGAATAAAAAAAAAAAAAAA
5' EST: CX814556 [+]tgcctgtctctggcgccgcaagggacgcagctctgctggtgacaccgtattggggggaacattggtgacaggaagagcaaggaaagtgacagggacaccg
gttgctaagcggaccagcagatccttcacgccagagaagatcacgagaggaagagccaagtgatacagacactgatgctggtggcagctggagacggaca
gacacagagacggattcatctcttgaaattcacgtcccgggggtacagagtcggctggcacagacacatatactttctatcttctgttctgtctcccttc
atcaattccatctatgctctgtttccttgattttatagaggaagaagagagactacaattaaagtactgttgacagcaaacaaggtgcaggctaagtggc
catataacctgtgtacgatttttgtgcaagcatccttcctttcaaaagataatgtgcaaaattttgttgaggaatgtaatacacgggcttcgctaagttg
tggcactgagtttttcccaactgtacgcttcattttttccaactaaaaaattatttttagtaccacaatgtcatgctggtggctttggctgacaaccgtc
tttttcttttgccacctgcctactggtgcacgatctgattgctggctgattgagggggaaaaaggatatgtctggctagcaatctgcagccagaaccaac
ccccctatgagaccatacctcagcacatcaatagcacagttcatgac
3' EST: CX814555 [+]cttaaggtaaccctattattttgcaccatttgtgaactgaatttcatcatgcttttcaaaataaatacacaaaataggttttcaaacatatttaaaaccc
tgaagtgccttattgctgtgtgaattcagcttacgacagctttgagggggaataaaagttaaacttaaattaaatatattaatggagagaaagccactag
tacagtgtgtgaaatggagggggtggtgttttaaaggggggagaactgtgcctgaaaatgtcctctccaacccgcaagtccatctttctcctccccacct
ttatgaggtacagccccccacctccaatgtaaactttaattgcaaactgtgatacgtcaacaccacagttctcaacaaaaaactgtcaacttccctgaca
tccttttataccttttacaccttttaaggtgccagtcacagtttttgttgtgcagatacccctttccagtaatccaaaatatcatgcaagtcctcccctt
tagcaaactggacttttttgcgcagagcatgtccagctgctatatagacctcttcccgggtgtgttttttgtaaggacggaagcgctcccagatcttgtg
ggtattttcagtggagtactctgggcttgaggagtatccagagaaatggtgtctaggagacagttgggagtagctggagtctcttttagagcggggaagg
ggttttaggaagctgaccctctggcttaatgtatcagcgctttcctcat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
