XB-CLONE-3402834
|
Clone Name: IMAGE:7641101
|
Gene:
tubal3
,
tuba1c
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX794629 
[+]
attgtgggataaagtaacgtattttatttcatttttttaaatttttttcttgtttcttcgttgacttttaaccatgcgtgagtgcatctctatacacgtt
ggccaggctggtgtgcagatcggcaatgcctgctgggaactgtattgtttggaacatggcatccaacctgatgggcagatgcccagtgacaagaccattg
gaggaggggacgactcattcaacacctttttcagtgaaactggagcaggaaagcatgtccccagagctgtgtttgttgacctggaacccactgtcattga
tgaggtgcgcacaggaacataccgtcagctgttccacccagagcagttaattaccggaaaggaggatgctgccaataactatgcccgtggtcactataca
attggcaaagaaattattgatctagtactggacagaatccgcaaactggctgaccagtgcacaggtctccagggtttcctggtcttccacagctttggtg
gtggtactggttctggattcacctccctgctgatggagcgtctctctgttgattatggaaagaagtctaaactagagttctccatctatccagctcctca
ggtctccacagctgtggttgagccctacaactctatcctcaccacccacaccaccctggagcactctgactgtgctttcatggtagacaatgaggccatt
tatgacatctgtcgcaggaatctagatattgaacgcccaacctacaccaacctgaacaggctcattagtcagatagtgtcttctatcacagcctctctg
3' EST: CX794628 [+]tttttttttttttttaaaatatattttattccaggcctgacagcacattgatggtataatgacatgttaactaaaatatggattgtcaaactttgattcc
acttatcagctcaggttctgcttaacgtaaaaagtttaggtgtttagctgaaatccgatggtcaaatgcatgcctctatagcatttcaaggtctggttta
atattcttctccttcctcttcaccctctccttccacagagtctacaccaacctcctcataatccttctccagggcagccatgtcttcacgggcctcagag
aactcaccctcctccataccctcacccacataccagtgcacgaaggcacgcttggcatacatgagatcaaacttgtggtccagacgagcccaggcctcag
caattgcagtggtattgctcagcatacacacagcacgctgcaccttggccaggtctccccctggaaccacagttgggggctgatagttgataccaacttt
gaaaccagttgggcaccagtctacaaactggatgcttcgctttgttttaatggtggcaatagctgcattgacatctttgggtaccacatcaccacggtac
aacaggcaacaagccatgtatttaccgtggcgtggatcacatttcaccatctggttagctggttcaaagcaggcatttgtgatctcagcaacagaaagct
gctcgtgataagctttctctgcagagatgacaggggcataggtggcaagagggaagtgaatacggggata
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
