XB-CLONE-3476539
|
Clone Name: IMAGE:7744163
|
Gene:
actg1
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: DN037235 
[+]
aaaccaacagtacaattaaagaatggaagtacagtgctctgtgcataaagtagtatatctaattcatgtacaccccttaacctggattatgtgcagttcc
gagtaatgtacaaatacatggcttagaccgcacacagttgttgtatagtggttttctgttctaatgtgatgaatgcattctctgtatcatacatccatgc
tctaatagtctccaaaatgttttagaatagtaatgaatatgcttgtatggctcaaacttacctttaacttgttttgctctgttagcatacccttactttg
tgcatgtctggctaggaatcaaatggatctttgtgcttcataaaatttccttatgcccactatcaaccaacaaggcacaggacgcgtttctttgcatggg
tgaaggaaagttaaaagggatttacactatgcttaggaatcagtgtacagcactgcctaaatataactgtatagaatatgggcccataatatatactatt
ttacaacttgaaaatacatctgtgaaagggcaataatataatgccaccacaaaacagttttacagaatcaaacgtctcagctttgtgtataagagtagta
ttcattacatttgaattatatattgtgtggagtgttttctgtataaattgtgagccttcacttcacactttaaacataactggctgcggtattagcaccc
tttggcatatggaatggcaacgttaatcttcattcagaatagttccataggattcctgtacataagcaacttaagttccttaaaagtttctttataaatt
taagttatgggttacatgcttgaaaagcattatatttaaa
3' EST: DN037234 [+]cttttacacagctgtttaatttttcaaaggtaaaaagacaaatgggttacaaaaaggtataaatgaatatcgtatcaaaatcagaaaacctcaccctttc
acacgccaatgtctgctaaactcccagcatgtacacttgcttttcattgttaaaataacctctatttacaatttaaaactgagtctaatatacaggcaat
tatactggctgtcatacattcattctttctatatttgtaattgtacatgcacccaaggcctgatctactccatgccttgactggcagtctccatacacag
cagtggtaacagcacctcgtggttgtccgaaacagatgctgccaacattgtactaaagtaaaaggaaaactataaccctcaactatgtacaaaatataat
aacataaaacagcttgtatgtaacaccctgctttatctaaataaaccaatggcacacaaacaggtcacatcagccaattaatggacatagctctgccttt
tgctctcacacttcttcctgctacagtcagagctgcattataccctatcaggtgatctttgagagaacagaccatcacaaaatggtagctcaagggaaaa
gatgtaaaagggcaatattaacacacacatatatacagtatatcccagtttgataggattctttaataggccacttaatatgatataaactatctgttgg
ttaagtattcattctgggggtatagtttccctttaatgaagaatgcat
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: liver | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
