XB-CLONE-3483708
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Clone Name: IMAGE:7741621
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Gene:
gck
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Species: Xenopus tropicalis
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Sequence:
5' EST: DN032283 
[+]
ccaggcagcacttctgtgatccagatcaatgaccattcgtcatgtatattacacagccatcaaaatgtgagaatgaactaaatgtatcaatagccacacc
agcagccactgttcctcctaaacttttcaagcatcaacattaaatcatctaaaagcctcccatcacgggaacatttgtgaaatgtcaaaggctgtcaaga
aatacagaaaaggacagaactgcatctcagtatattaaatgaaacacgcagattgcttgtacagttatctataaatatattcactgaacctagtctatat
aacatattggggcatcatctgatcatctgttctgaaatcagttgacatttttggctagatttcgccactgactattactcaggtagtgatttgtaaaatt
ctctactacccttcaggaacagtctatggatagaagagacacaggaggaatattctgtttacagtaaaagacaacctagactattctactaacagacaaa
tagacatgaaacttggagctggactttatcagaagccagaatgtgatgaagtaattgggaatgatgtcactaaacaaagtctttgtcattggtgacaact
ggaacatgtgatgtaatgaggaacacagtgtcagtaccaagaaaattgtatttacattaatatcattattgtgccagtgatatgtatgtcacaatggcaa
tacgcaggcagtgaatgttttctcatataaataatattatttaggctcctacagaaattactgctgcagaaaagcctgctgctgctaaagacagatataa
ggactttaaaataaataaaaattggaaaaggacctcatnnagaatagcaatggtaatgga
3' EST: DN032282 [+]gcaacatgacatatttattatttccaacaaataaactgataccaattaatacattgaacatataatctctaattttgtggacaacataacaataaaaaca
aaacagatatataattctgctaaattaatacgcataaaatatgtcattaaaccagcatgacacagatgacctgaaattctggatcaagttccattttagt
tcttggacaccttgtccctcagtagaagatgggcaatttgcccaaacaggaatgctggtagatgtagcatgctgggacccatggtctgcatctacactta
acaagagaagaagaacagtctacatggggacccacataaatgagtctggattgtattcattattaccatccattagcaaacccttatatttatgtggatg
ttgccttaagatttgacaatggctttgtggcttacgtgaaagtgaaataaccgacccctcgtctgtgcatgcactctcattcaccattgtggtatcagtt
aaaaaaattcacatccagaagaaagcaatcccaggcaagtcttccattaccattgctatttcctatgaggtccttttccaatttttatttattttaaagt
ccttatatctgtctttagcagcagcaggcttttctgcagcagtaatttctgtaggagcctaaataatattatttatatgagaaaacattcactgcctgcg
tattgccattgtgacatacatatcactggcacaataatgatattaatgtaaatacaattttcttggtactgacactgtgttcctcattacatcacatgt
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: liver | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
