XB-CLONE-3493244
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Clone Name: IMAGE:7737714
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Gene:
vcl
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Species: Xenopus tropicalis
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Sequence:
5' EST: DN025718 
[+]
tccaaatcaggaattcaaaaataagcacctgcaaccactgggagcaaaaggtaacccatggcaaccaatcagtatttgttttcaaagagatgacctgtaa
atggaacctgctgattggttgctatatgttatcagacaagtagcaaacttagtaccttttttacattactctttagtgcttattctatagcatctttcta
gatgttgctgaactagaattacaaactcacaaaatattacagaaagtccagaataggtcatatgaccattacagtacaagaagatagttatgttaattgt
gaaggatcagaaagactgcagagtttatataatttcttaagtatatgtttttaaacaaaacacaccagtgctattaaagaggagttgatttagaagcatt
tcattgcatgtgtcagtgtttatttaatcaaaaaagttacactgaatgtactaaatatatcattttactctgggatctaaatttttttcagtaaacaatt
gtcatagactatttaatagtcttaaagtaaacctaattgtgcctgaatattttcatataacttgtattaataattttatgctttagagggttacaaataa
tttactataagtgcactgagatcagttattagcagtggctattgtgttatttttttaaacataatttacttataaatctgttaatttaatgagtgaattc
ctggaaagcataaagtgggaatatcttatacatgcattgctagaaggcacttgtaatggtaagatttacctgtaa
3' EST: DN025717 [+]ttttttttttttaagttgaatacatgtttttattattgccttttgagatatttgagttcactaaatattgatagagcagttaagggataatatgttatag
tacattaaaaagaaaaatgacaggcaaaatgtaatgctggctgtgaagcataatatatttgggcttcatctgtctcttgaaatactctttatgtttacat
atatgtggcccacacaaacaaaaagtcatgacagccctgataaaaccaatgctttttttaaaataggtagccagtcctttattggatacttttgaaaaca
gaggtaaattttatttgaaatggacacctttagatggtcaggatctttgatagttgactacaaacatagcattccaaaattaacaaaatatacaaaatat
taacttggctggctgtgcaataacagagagaagttctttggtgtaaattttaggtgtgcataggttaaaggacatgaaaagcctcagtcactagaagtcc
caagttgataggccctcagtgactctaatcgctaatatggtccgccaactcctcctcttaaaaaaaaaaatgcacagggcaggagtagttttctgcaggg
caagctacaaccatctttcctcagcttcccaggcattccctaaatcatgctttaggctttgtgcctgagtagtatagaatttaaaaaaataaaataaacc
tgatcttgtgcaaggatgccagaaaagctgggaaaagaagtgagagccatcctgcacaatctttccccagactcatgcagtttttttt
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: liver | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
