XB-CLONE-3502295
|
Clone Name: IMAGE:7734523
|
Gene:
hif1a
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: DN019439 
[+]
cacagctccggagcgtccctcccccactcgcttggcatggagggatctgtagtagcgagcgacaagaaaagaattagctcagagcgccgtaaagagaaat
cacgtgatgctgccagatgtcggaggagtaaggagtcggaggtcttttatgagctatcacacgagttgcctttaccgcacaatgtcagctcgcatcttga
caaggcctctatcatgaggctcaccattagttacctgcggataagaaggttgcttgacgcaggagatttggatggtgagaccgagttggacaagcaactc
aattgtttctatttgaaggcactggaagggtttgtcttggttcttactgaagagggggatatgatttatctatctgaaaatgtcaacaagtgcatgggcc
tcacacagtttgagcttactggacacagcgtgtttgatttcactcatccttgcgatcatgaagagctaagggagatgctaactttcagaaatgggcctgc
aaaaaaaggaaaagaacaaatcacagaaaggagtttctttcttcgtatgaagtgcaccttaaccagtcggggaagaacagtgaatataaagtcagccacg
tggaaggtacttcactgtacaggacacatgcgtgtatatgatgcaaacaaccataaccattgtggctacaagaagcctcccatgacatgcatggttataa
tctgtgaaccaattcctcatccatcaaatattgagttccccttggacagcaagacctttcttagtcgccacagccttgacatgaagttttcctattgtga
tgaaagggtaactgaattagtaggatatgaaccagatgaacttttggggcgttctgtctatgaatattatcatgccttggactctgatcattt
3' EST: DN019438 [+]gtggcctggtccacgtataatgtttattgatgtaataaaacaatacagttagtgttagatccaaccacaaacagcaaaaaggataaatggaaaacaattt
gcacaaatctgtaatgtacaatcacactgaagttgttcgatgttacagctattttactgatatttttttttctctaaaaagtcataagaattaaactttt
ttttttaatgaaaaaataaagatgttaatagtgggaaaagtgcatgaaatcaaataccaagcatctctgtgcactatgcaattctgctaacaatgcgccg
tctctgaatcagatggattttctgtccctaagttataggcagaacatatctattataaacgcctatgcaattagcatcaaagccttgaactacaagcagg
gattctatgctgttatacagtctacatacattttaaacatgtactcgtaaaaaagaatgtctaactaaatgattttaatcactctagttcatttccatct
caaacccacttgtaattatcactttaaattgacactttagccaacaatctgcaacagacaagttataacttacatctaacaatgaaaaatctcaggcctt
acatgattcgaaacaactaaaaagaatccaaaacattttataaactagttttaaaaatgcataatatatattgcatgaggaactgctggtattccatttg
ttgttttcttaagaaaatataaattgtgcgattgcgactacaatgcaccgctggttaagcattgtttgctgtgcataaaaagaactgaaaatgtatacta
aacattcaataaaatgacaatgaaatggtacacatggttggactga
Library:
Description: The library was prepared by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: liver | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
