XB-CLONE-3537038
Clone Name: IMAGE:7720460
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Gene:
epn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995012 [+]
cagcttcgttttctgatccatggggtggatcaccttctaagccgagcacgaatgggacaatgggtgagctagatctgttggcaggagaagtccctatgtc
tcgttctttaggatcaaagagccctgatacctttgacatgacagcaatgagtggctcactgtctgactcctgcaaaccaacccggaaaacacccgagtcc
ttcctgggacctaacgctgcccttgtagacttggattctctgatctctaaacctgcattacagaatgcaaagacctccaaccctttccttgttacaggaa
cacccaatgcaaccacaacaaatccattccagcccaaccaacagtcatctcttactctgaaccagctgcgctcaagccctgtgatgacattgggacagcc
agttaccccagccggccaaacgcccgcgaccattcctttcacttcccccatggtgtctgtgtctcctatggcccctggaattcctatagcaaatgttgca
ccaatggttggaatgcagcctatggctggagttcccaacttgccaattggtaccgtggctcctggtgtacctggtatgggtttgccacctatgatgccac
cacagcagttggttgcacagccacttgtcccaaatttatcaacacaagctgtgacgagtactactaatccttttctgttatgaggtggggagggggttaa
agtatttgtagaggtgatgaccaggtgtgatgggtctagaatggtatcttttacaactactacagtccctgtatcctcgcaaccctgtgtaagcagcaaa
atgttttctggtgtctgcttctcttttgactgtgc
3' EST: CX995011 [+]attcagtatacatcccaaacctaggcataactgtgatgaaagtgctatgaaccaaagagtggtattaaaaaactgatagcctataatgtcagctaatcat
atggttaataagagttcatatgaaacaaactgctgctgacatacagtgactgcagttaccctttaatactcaaagataattgttatgggctggactgcaa
gaacgatgggaagaagtaattacatatatgtctgctgcatacaagcctctgtcagaggagatgcgaattaaaaagatgccaggaacatttgcatcaaacc
tggaaacttccagggggtggggggtaaaaaaatcaaacaggacaggttacaatcacagagtacagttaattaacgcaaaaaacgatgaggggtaaagggg
atcattgtagagttctggcatagttctcatgatcatgaatttgcagaatgcaatattgaataatggtcttctacccaaactccagttgctgtactgctcg
gggttaaatactaattcttggtatttggaggcacagtcaaaagagaagcagacaccagaaaacattttgctgcttacacagggttgcgaggatacaggga
ctgtagtagttgtaaaagataccattctagacccatcacacctggtcatcacctctacaaatactttaaccccctccccacctcataacagaaaaggatt
agtagtactcgtcacagcttgtgttgataaatttgggacaagtggctgtgcaaccaactgctgtggtggcatcataggtggcaaacccataccaggtaca
ccaggagccacggtaccaattgggcaagttgggaactccagccataggctgcattccaaccatggtgncacatttgctataggaattccaggggccata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: