XB-CLONE-3537609
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Clone Name: IMAGE:7720263
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Gene:
stxbp6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984045 
[+]
ccgaggaaaaataccatcccttatgtatctttaccagatatgaacccctttattgctttagatattaacatggatcaaattaaaatgtgcgttgctcaac
cgctggcgttgctcaacctaaaacatgataaaatgggtcacaaaagtccaaaaagcgccttggtctctctcttgcacacagaaagggaatgcataatttt
actaagtgctaggtgctggcgcacctcctatttatggaagcagcggggcgttccctgcatttggatgcttgaacaatcccaatagtcagtccaatgttcc
aacactgcacaaggaacagcatcaggtacagataccccaatattgtgcaccttgaatcagccagtgctctcctacggtgcaatatccccaatcatgcaat
gctcatcccttcacttaaagtgtgcagtctcatgtcatgtgatgtactctgcagtctcaccctcattgtaatttgctaactggctgcattcaatgtattg
actatgaggattcattaaaaaaacagttccactgcttacaaatccttcgtgctgcttcagaccttcacataccctgcatttgtgactgacagtatactca
tctcagaggctacagggccagatgtgccaaaactgatctgtcaaacagcaacgagatttcctgcagagatccgagggctgagggtcatatccagagctga
ttctccccccagagtccggtttatccgatggaaagattacactgtcggcagtttagcccttctccagaggacttagggatgtgcagcagcaaacgctggc
caaagtggcaggtgtctcgtttaaaataaaagatgta
3' EST: CX984044 [+]cagctgaggcaagttaatggcactgacgccaacaggcaggagtccccagagtttgacctgcagtttgacaatgcttctgatcagtgggttgcaagttctg
gctcggagaaatttaccttctttcagatcctttaccacacgtgtcagaggcacctgtctgaccgaaagcctgaattcatcaactgtccgtccaaattgtt
ctcaggcaacagtattcttcactctgcagccgacagcgtaactagtgctgtacagaaagctagccaagccctgaatgaacgcggggagcgactgggccgc
gcagaagagtcaacggcggaaatgatgaatagcgcccaacaatttgcagatactgcgcacaagcttgctatgaaacacaaatgctgacacttgcacgggt
cagaggggggacctcctgccagaatatgccaaaacctcagcacaagattattacatcttttattttaaacgggacacctgccactttggccagcgtttgc
tgctgcacatccctaagtcctctggagaagggctaaactgccgacagtgtaatctttccatcggataaaccggactctggggggagaatcagctctggat
atgaccctcagccctcggatctctgcaggaaatctcgttgctgtttgacagatcagttttggcacatctggccctgtagcctctgagatgagtatactgt
cagtcacaaatgcagggtatgtgaaggtctgaagcagcacgaaggatttgtaagcagtggaactgtttttttaatgaatcctcatagtcaatacattgaa
tgcagccagttagcanattancatgagggtgagactgcagagtacatcacatgacatgagactgcacactttaagtgaagggatgagcattgcatgattg
g
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
