XB-CLONE-3552288
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Clone Name: IMAGE:7714604
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Gene:
krt19
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973989 
[+]
cccctatgggagctggcaggatgttcgccaaagccccaagtgtccatggtgggtatggaggccaaggtatctccgtgtcttctgccaagtttgtttcctc
tggcatcggtggaggccttggcggtggcagctatggtggtgggttcagcagtggatatggtgctggttctggatatggtgctggttctggatatggtgcc
gggctcagcgtcaatgtatccggggatgtcctgctgtctggcaacgagaaggagaccatgcagaatctgaatgaccgtctggcaatgtacctggacaagg
tgcgcgccctggaagaagccaattcaggcttggagctgaagatccgggagtggtatgaaaaacagggacccagcccttcccgtgactacagccattactt
tgctatcatcgaggacctcagaggcaagatccttgacgccaccgtcgacaattccaagatcgtcctgcaaattgataatgccaggctggctgcggatgac
ttcagaaccaagtatgagaacgagttggccctcagacagagtgtggaatgtgatatcaatggcctacgcaaagtcttggatgagctgacattgtgtagaa
ctgacctggaacaccagattgaaagcttgaaggaagagttggcctacctgaagaagaaccatgaagaggaaatgaacgcaatgcgaggacaagttggcgg
gcaagtgagcgttgaagttgatgcggcaccaacggtggatatgaacaagaaactggcagaaatgagagaacagtatgagctcctggca
3' EST: CX973988 [+]cgaatttctaaagcatcatcatttcctagggattcctggcttcgtggtgctgggtatttgcactggcagtggaaagcgtaacagatccctgtgtgatgga
ctctccgtaagcgttttctatgcaatgaatgaagacgggcttgattgcaggctgtctcagctccaccagtggcctcacttccctcctgttttagtgtgca
tcgtgcccttctagcagctgtcggtaggtgctaatctctttctccaggcgggtcttgatgtccatgagcaacttgtattcgtggttctgccgctccatgt
cgctccggaggtctgcgagctgagcttcaatgctgccgatcacgttctgtatctgcgctagctgcattccgtaacgggcttctgtttccgaaagtgtgtt
ttcaagggctgctttcatgctgagctgtgactgcagttctatctccaggccctggattgtgcgtcgtaggtctgtgatctccgatttgctggtctgaatc
tgttctgtatgagttgcaactactttgttcaactcctcagactgtgagaagaaccatgcctctgcgtctctgcggttcttttctgccaggagctcatact
gttctctcatttctgccagtttcttgttcatatccaccgttggtgccgcatcaacttcaacgctcacttgcccgccaacttgtcctcgcattgcgttcat
ttcctcttcatggttcttcttcaggtaggccaactcttccttcaagctttcaatctggtgttccaggtcagttctacacaatgtcagctcatccaagact
ttgcgtaggccattgatatcacattccacactctgtctgagggccactcgttctcatacttggttctgaagtcatccgcagccagcctggcattatcaat
ttgcaggacgatcttgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
