XB-CLONE-3554449
Clone Name: IMAGE:7713961
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Gene:
zfp36l2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972475 [+]
cccatgtgtataaacccttaagatggaggtgaataaaacattgcagaagaatgggttcttcctgatgtgagtttgatcacaaacatttaaatgaatctca
cgcaggagtcaacaattccttctgataataaactaaatatcaacaatacccttttaagaatgaatggaattagttcacgggattggctgattgttcttta
tttttagccaaatctttggttattataaatgttaaattgtctaaaagagaatagggttcttaaaacaaataaaccattcctttcttttttaaaagtcaaa
tatatggatttatttttgtctgtcttagaactggaaagataatatttagcagtagggtggttgaccaaatgtacatgaagagctcattgttcatatcatg
agtcaatgaaaagatagattatgtaggagtacccctcctgccccctacctgtcccaacaggtagcaaatgctaagtgagcactgaggggagtgctcttct
gccattatcactcaagcacttgtgccatggggtaatgtaaatatactctttgtgtttttaagggcagtaatctaagggtatgactacatgggagattttc
agtttttgtgggtcagactttattatacagaagcatgcaacagcacaacttggtaaaaatatttcaatgagcaaacaaaagccacaatggcagccatgga
ctattgaattgtgcaaattggcactattcattaaaggagaagaaaaggtgaaatcatgacattttaggcatccccagtgattatattcccttatctgata
tcctgggctgttag
3' EST: CX972474 [+]gtgacataaaaacatcttgtctgagaaccaccagccacttaccaatattaaaatacatacataactgcagccaggtgggtaaataaattaacacgcttat
gtacaagagaaaaatgaaggatctaaataaaacaaactggcttctcactgctggcatagaagtgaaaaactggggaaacatgctatgcatgttaatccta
caccagaggagggggtcattgtgccagtgtcatgaaattaaaaaaggtaaattttagtcgagaacagaaagtagaccctgggcaatttcttcatctccag
gcagtgggtcagtagcagtaggtaagtcttcctcatctcccaaaatctgcagacgcagggcaagaggtaggagaaggcttgcaaaactgaaggcattatt
agcaggagattctgagaggacaggagtgccagggtcactatcggggtctggatctgaaggtgagagggacccactgctgttctttgttcctgaaccccac
ctgtcaaggggagggctatgagagaagtggcaacgggctccatatggacagtctccaagagcagcaaagtgacggcaaacctcccgactactttttgggt
gctgaaagtggcaccgagctccataaggacagccaccaggagaacgccagaggcggcaccgctccctataagggccagcatacttgcgtgttggaagacg
aggggcatcaggngagactggagactcccttctctcctgtgggctgtgaatgaacaaacaacgcaatccatagttacaggtaccaagcacgtggaaggag
cgacacagttctgtcttgtatttagggtgctgaactggaggcctgagctcactt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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