XB-CLONE-3555134
Clone Name: IMAGE:7713658
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Gene:
abcc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972006 [+]
agcatgagtaaattacatagtttactaaaaaaaatccttctgtgatcatttagattagaaagaaagatttagctttggttacaaggttaccctgcagcaa
gaagcaggtgcattaacttgcagtgggactgctccatcaggccggcatccggagccctttattcagtctaaagagcaatcagaaccttcttagctgctgc
agccataggttagtctgtgtcagagcctctctgaatgctccagtaccagttggtactacgaaacctcttctaaagtctcccaaataaaagtattttggtg
tcttctttcccatatttatagtatggtactttgagtgctatcaatgccagcatccttggccatagaaaagaaatgtccctggcgctgaagaagctcatct
ggacttccaaattctatgatcttcccggcatccagcaccattaacctgctgctgtccataatggtgtgcaaccggtgcgcaatagtcaggactgtgcagt
ctgcaaactcgcttcgaatcgtcctctggattaaattatccgtctccaaatctacagcagccgtggcttcatctagaattaggatcttagatttccgaag
caaagcccgagcaaggcatacaagttgtctctggccaacgctgaggttctctccaccttcactcacttcatggaaaagtttctcctgaagcccttccaca
aatggcttgagatgggagagttcaagggctttccagatatcctcatctgtgtactggttgaatggatccagattcatccttaagttgccagaaaacaaga
caggatcctgtggaataatggtcagtttttggcgtaggtcatgaaggccaatgctttgcatgtccagcccatcgatcataa
3' EST: CX972005 [+]caacagggcgcattgttaacaggtttgcaaaggatatcctaactgtggatgagaccatcccaatgtcattccgcagctggatcgcatgctttctgggagt
tattggaaccctattcatcatatgtttggctactcccatcttcgctgctgtcattgtgccacttgcaatcttctattactttgttcagagattctatgtc
accacctcccgccagctgagacgtctggattctgtcacaaggtctcccatctactcccattttggggagactgtctctggactgtctgtaatccgagctt
atggacatcaagataggtttctgcagcacaatgaagataccatcgataataatcagaaaagtgtatattcttggattgtctccaacaggtggttggctat
tcgtctggaattcgtaggaaaccttacagtcttctttgcggcccttttcgctgtgctgggacgagggactgtggatagtggaaccgtagggctttccatt
tcttccgctctcaatatcactcaaaccctcaactggttagtgaggatgacgtctgagctagaaaccaacatagttgcggtggagcgagtagatgagtata
taaaggtggagagagaggcacagtgggtagaagaccatcgcccaccaaaagactggccagagaaaggagttgtacagtttgtggactacaaagttcgtta
ccggcccgaactggaccttgtcctgcagggtatctcatgcaccattaatagcatggagaaggttggtattgtcggcaggacgggtgctggaaagtcctca
ctcaccaattgcatctttcggatcattgagtcggcgggtggcaagattatgatcgatgggctggacattgcaagcattggccttcatgaccta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: