XB-CLONE-3556201
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Clone Name: IMAGE:7713176
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Gene:
ybx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971283 
[+]
ttgttgactcttgaaaaaaattaggtgtacgcttattacaaattaaaaatgaggttcttaaaaatcttagtttgaccagataggaaacaatttaaaaacc
tttgaaaggtacattgagaaaaaatatgtttttaaaaaattttcattcccaaaaggagcttttagaaaaaaaacataaaaaccatctctgcatttaccca
tgcaggatgaagttatctggtcaagagcaaaaagcaagcacttaaggtcttcagttccaattctttgttcatttcttattgctggaatttctgtcttgac
attttgatgacaaaaaaccggatggtggtagagttggtaagccggtatttactcagccccgccctgctcagcctcgggagcggacgtgttctcagctgat
gtttcggctgccttggtctctttaccatcttgtggtttagggttttctgggcgtctgcgtctgtagttaaagttacggcgataccgacgttgaggtgcct
gctgactctgggtttcatccccctgattttctttgtcctcttcatttccttcttctctgggttgtctctgccgtggtggtcccctgcgaaatcgtggtct
gaagcctctgtacatattctgtcgcacaggtctaccttgatcaccttgtgaagaaccttgaccatctgatccctctgcaccttctccctgaataggagca
tttgagtattgtggtctgcgcccatatggtctccgcaagtagtatggtggaacacgtctcctgcggtatggacgttgctgatttgaccccccctctcctt
caggtgcactctcattcccttctgtct
3' EST: CX971282 [+]ttagggcgagaggcccccgccggcctgagttaccatcaacaccccggggggaagttaaagcgagccaaccgccggcccgagtcaccacctccgaaagcag
ccaacttacaccatcaaagacacaatgagcagcgaggttgaaacacaacagcagcagacagtcgcattggagggcaaggccggccaggaaccggcggcca
ccgtgggggagaagaaggtcatcgcaaccaaggttttgggtacggtcaaatggtttaatgtgcgcaatggttacggcttcattaacaggaatgacaccaa
ggaagatgtgtttgtacaccaaactgccatcaagaagaataaccctaggaagtaccttcgcagtgtgggagatggtgaaactgttgagtttgatgtagtg
gagggtgaaaagggtgcagaggcagctaatgtaactggtccagggggtgttccagtccaaggcagcaaatatgcagcagaccgtaatcatttcaggcgct
atcagcgtcgcagaggtcctccacgtaactaccagcaaaattaccaaaataatgaaagtggagagaagacagaagggaatgagagtgcacctgaaggaga
ggggggttcaaatcagcaacgtccataccgcaggagacgtgttccaccatactacttgcggagaccatatgggcgcagaccacaatactcaaatgctcct
attcagggagaaggtgcagagggatcagatggtcaaggttcttcacaaggtgatcaaggtagacctgtgcgacagaatatgtacagaggcttcagacca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
