XB-CLONE-3562206
Clone Name: IMAGE:7710772
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Gene:
pnp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967094 [+]
tgctgctggaggtctgaaccaagagttcagtgtgggagacatcatggtaataaaggatcacattaacatgctaggatttgcaggacaaaatccattgttt
ggtcacaatgatgaaaggtttggccctcgtttcccccctatgtctgatgcatatgacaaggaaatgaggagtctgttgctcgccactggcaaggagctgg
gttttaataacatgagagaaggagtgtattgtggtattgggggacctaactttgaaacaattgccgaatgtcgatacctcagcaagataggtgcagatgc
tgtaggtatgagcacagtacatgaggtggttgttgcgagacactgtggcttgcgcagcctgggaatatcactaattaccaacaaagctgttatggactat
gacagcaaagctactgccaaccacgaggaggtgcttcaagctggcagagatagtgccaagtatatggagaagcttgtaagcactttcctgcaacacctga
acctaaatcaagtgtgacagcgaagggcacacactgagcacaaaggaataaatctactttcattttaatctttccctttctctaatatgtcagacctgcc
actttcagctcttatactgtaatgccaggattttggaggaaggctagaaaatgtggttctacaacagctgaaggttggcaggatagacacccctgttcta
cagatctaacaataacaggctgatgccacactctatctacatatgctatctgtacctttcttttaatacagttgcatcagaatggtgcacaggatcagta
tcttgnatccatttactggacatggagcatccatttgtcattccctactaacaatactcccttttatc
3' EST: CX967093 [+]ctatagggcgagaggaaaaaatcttgcaaaagagaggactacatttattgtgacacattaagagaatatactagaattgtactcagctttcctggacatt
acatgatttcctcttcccttaatgacataataatgcattgcagttattagctttcagtaaatacaggagtacaagcaattcgatgtgaaaccaactacct
gtaattctcataatgaccctgtttcatgtagaaccatatttaacacacatcctgatcctattgccattagcagctggctctgcagactgtttcaactgtg
tgggtccaagttctctgacaacacaatggttctaccatccccttcctttaaagctggtgtccatgagcacaaaggcacctaaatatacaataagaaactg
cacaccaatgattacaatagtttattaacttataataaagctatgttacacatgcaggtgctggaactcaatgtggatcagtgggctagcagcctgaaaa
aattaaaggtgcacattttgtatgttgtacaggattattgttccctagtctgacattttggaacaatgtagtgtgcacctttgtaaactacaacaggatt
actcgctggcatgactttgtactgacttacagccctgtgaccttttattgttggatttttcaatttaaatacacaatttttggggacaagtaaagcacca
ccttttttcaaactggatgaaacaatcatgtttcaaatggatatgtgtttcatataattattttttttatattatgtttggctcttttacattttgcgtt
cacggcattccattgggtgtaactgcctatgatttgttgcctac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: