XB-CLONE-3562649
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Clone Name: IMAGE:7710635
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Gene:
slc15a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966798 
[+]
tgagcattttctttattgtcatcaatgagttctgtgaaaggttttcctactatggaatgcgagcggtgttggtgttgtacttcaaatattttctgcactg
ggacgataaccttgccactgttatatatcacaccttcgttgccatctgctacctgactccaattctcggggccataattgctgattcttggcttggaaaa
ttcaagaccattgtatacctgtctattgtttatacagttggtcaagtggtcatggctgtaagcgctattcatgatctcactgatggaaatagggatggga
ccccagatacacttgaagtccatgttgccctgtcagttattggccttctcctcattgctttgggcacaggaggaatcaaaccttgtgtagcagcctttgg
aggagaccagtttgaggagaaccaggacaagcagagaagtcagttcttttctatattttatctttcaatcaacgcaggaagtcttctctcaactattata
acacctatcctccgaggacaagagtgtggtattcacagccagcagaagtgctacccactggcatttggtgtccccgctgcccttatggttgttgctctag
ttgtttttatcgttggaagctcaatgtacaaaaaagtttctccccagggcaacatcatagtgaaggtttccaagtgtattggttttgctatccgcaacag
atacaggaatcgcagtaagtcaataccaaagagagagcactggttggattgggcaaaagaaaagtatgatgagcgtctcattgctcagattaagatggtc
ttaaagtcctttttctctacattcctctgccaatgttctgggctctctttgaccagcagggat
3' EST: CX966797 [+]ttcaatattttgtatctgaaaacaaattatgcagagcggcgatctccagaaggtaagcgctggcgccaaatgctttccacttgatctctcacattgcact
ccagcgttatcattagtttgatttccaggcaacaaggtatttcccaaatgacaaacggtatatttacaggtttttgatatcatcatcccacattacattt
ttgtttgtgaataaggtccatctccattttgaacacccattgcctctatttcctttgcattctttttcttttcttcttcatcaaactgagcttcaatctc
ggcaggatccacataagtgtagaagtaggccatgacagcaaagatgatacaaacagctaccaggagagcagcaaagagcacatactctgcccactgttga
ctaatgtgtgatgccccagccacaataaggacaatgatatttccaacagctacagtcaacagccacccagcttggagaacagatttcatgttggaaggag
cctgagagtatgaaaactccaggccagtgactgaaaatacaacctcgccagctgtaataaggaagtactgtgggatctgaagagccatgtgaatagtgtt
gggttgaatgtcttcaacatactcaacttgtagttctgtgttaccctccttaatgattatggtatatgagctaccaaagccaaatttaattgattcaact
ttccatgtttttccactcgccgtanaaacttcaatttcttttattcctagcttttgcatggtataattggacatttgcaatggcatcagctgtcccagag
aaactgcgttgagtnngtacgtgataggttcttgcataccgttgacaaatctgatttgcgtgctgccttggtctggcttggcactaatgtcttcaacaca
tttgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
