XB-CLONE-3564168
Clone Name: IMAGE:7710202
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Gene:
tbrg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965764 [+]
taaaagtggggaaaattattttattatgacacaatggaacagtcatataacaaacagctttttcatgaacaagttatttccagtgaaaaaatgtcaacat
acaatgcaataaattatattttagtatttcctccttattctgaagacctggacggactgtactgattggaaggtgactttaaatgctgcatgcctgatgc
caattgagaatgtgataagaagatttcctggtaagtagatatataatcatgtgacgcatgtatttctggaagatccaaagttcctgctaaggcagtacta
ttgtttattgttgcaaaactttgtctttgaaaggcttcaaagttaacggaggcactgctttcacatatgtcacgaggaacttgctcttcaccagctctgc
atacttcaaatttcacccactggtaacctgtacatttccgtgctccagggcaactctgtattaaattctgaatagtaggatgagtaaatccaaagaaata
tgctccagcaagatccggtgtagaaaaccttttccctaaaggggtgctgattttctgcagaagaattgaatggcattcgtcggctgaactcgccacaatt
gaattctgaggatcatcatcaggcacaatctcaaactgcggtcctgtgccaccatcttttatctggcaagtatagaggcatttttgatcagggttcttca
tgccaacgtaaactctagtgctgcaaaagcctacaggataaatggcaactttttcatgaaatcctgcacggtctgaaataatctcaccaaggctataaac
cgtcagtccttctaatactattgggaacacaggccttccacatggatctaaagcaat
3' EST: CX965763 [+]gtccaccaaaaaaagccataaagagaagtacagactaaaatgtctccgtttaagacgtgtagccaaagatatggtgtttgaaaatgctgctctttgtgat
gaaatagcacgcactgaggacaaatttatcagagcgaaggaggaaagaaggtttttattaaaaaggctgctacagttgcaggctttaagtgaagaagaac
caggcacatcacataattccaatgtgtcagctggttacagtgtaccagacatggcagcgatgaatgaaggaaatcttgatatgtgctattcaagtgttct
tgatgatggaggttcatgcaagaaggtgaagaaggataaaagagaaaagggaaaggaaaacaaatcagaagctatgaagaaaccttccaagaaaaaaaga
gtcacagaaggaacaactagaaagtgggttcaacctattgctttagatccatgtggaaggcctgtgttcccaatagtattagaaggactgacggtttata
gccttggtgagattatttcagaccgtgcaggatttcatgaaaaagttgccatttatcctgtaggcttttgcagcactagagtttacgttggcatgaagaa
ccctgatcaaaaatgcctctatacttgccagataaaagatggtggcacaggaccgcagtttgagattgtgcctgatgatgatcctcagaattcaattgtg
gcgagttcagccgacgaatgccattncattcttctgcagaaaatcagcacccctttagggaaaaggttttctacaccggatcttgctggagcatatttct
ttggatttactcatcctactattcagaatttaatacagagttgccct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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