XB-CLONE-3564589
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Clone Name: IMAGE:7710113
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Gene:
fa2h
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965475 
[+]
tgtgaacctccatcctggaggggagcgattactgcaagaccgggctgggaaggatattcaagcagacttgaatggacccccacatcggcactcggaaaac
gccctccgctggctggagcagtattatgtgggggacttacaggcagagagcccggaacataagccgcagaacttgaaagagaaaccgcaacaatacagct
cagccacagaccataaagaagattctactggaattcagtttaaaactttcagcccagagacggatttagtagactgggagaagcctttactctggcaagt
gggccacctacgggagaagtacgatgagtgggtacatcagccggtgaaccgccctatccgactattccactcggactttgtggagtcgtgttctaaaaca
gcttggtacatagtgttgtctgtatgggtcccagtggttctttatttatcctggtactgtctcacagaattggctcaaggaaacacaagactcttttctt
cattcaccaaagattattctgttcctgtgccagtgtttttcttctttccacttttccttattggagtattggtgtggactctaatggagtatggaattca
cagatttgtcttccacatgaacccacctgctagtaactactatctcatcaccctgcacttcatgttgcatgggcagcatcataaggctccctttgacagc
tcccgtctggtgttccctccagtcccagcatcctttgtgattattcctctgtatatcttggtgcagctgatttttccagttccagtggggctgtctgttt
ttgtaggaggactttttggatatgtggcatatgacatgacacattattatttgcactatggcagtccctaacagggctcttacctggc
3' EST: CX965474 [+]ttaggcatttctacacatggattttacaatgtgctgttcacatgacaggggaagccagggttaaagcttaagtctgtcagtgcgattttaaaggagaaca
atgtgtatgcgtgcctcctttgtcaaggagtcagggtccatttcaactatggaaacacttgcattgcattagcttaagaagatgccaagcctgggtgttc
ttacattttttggcaatcccctttaatttgtttttccttaataaataattatttcccgctgacatgcatcccagagcacatgggatcaatggaaaaagtt
gaacatcttttatgtaaaaaatgtgaaaacacatgttctcctcctcctttatgtacagtaggctgcaagccagcaggtgtctttctgacaatgtatagct
atgaggatggtacgttctagctgtctttatccttatcctcaggaataagagtgttgaaggggcggtcccacaaggtgctggtaatgccaaaaccagattt
ttgatgctcgaagtgatgacggacatgatatgactttagccaggccaggtaagagcccttgttaggactgccatagtgcaaataataatgtgtcatgtca
tatgccacatatccaaaaagtcctcctacaaaaacagacagccccactggaactggaaaaatcagctgcaccaagatatacagaggaataatcacaaagg
atgctgggactggagggaacaccagacgggagctgtcaaagggagccttatgatgctgcccatgcaacatgaagtgcagggtgatgagatagtagttact
agcaggtgggttcatgtggaagacaaatctgtgaattccatact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
