XB-CLONE-3577941
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Clone Name: IMAGE:7695445
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Gene:
dennd5a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166235 
[+]
gcaggacagctgtccggtgggctcccgtgatgcagcgcgcagagccgtgtagccccgggcccccggaggaggatgacaggcgggagaggagggcggtttg
acagcagcaccatgagcgggggcgggggaggcagcagcacggccccgggccgtttcgccgattatttcgtgatctgtgggctggatacgggcaccgggct
ggagccggacgagttgtctgcattgtgtgagtacatacaggcttctaaagccagagacggggctggacgtttcttacccagtgcggctgaaggagagaat
tttgagcagacccctctgcgccgcacctttaaatccaaggtcttggcgcggtacccagagaacgtggagtggaacgccttcgaccaggatgccgtgggca
tgctatgcatgcctaaaggattagcatttaagactcaggccgaccccaaggagccccagtaccacgcgttcatcatcacgagagaagacggctctagaac
atttgggttctccctcaccttctttgaagaagtcaccagcaagcagatatgcagcgccatgcagaccctgtaccacatgcataacgccgagtatgacatc
atccacacgtcggcgagcaacggcagagactgccccggagagtgcaccgacagctccatcaccaagctgcagcgcttcaactcttatgacataacacgag
acaccctgtatgtgtccaagtgcatctgtctcatcgcccccatgtctttcatgaccgcctgtaagaaagtcctggagcagctccaccaggctgtgacttc
cccacagccgccgccattacccttagaaagttacatttataacatcctctatgaggtgcctctgcccccggctgggaggtccctgaagtttaatggggtg
tatggaccaatcgtgtgtcagaggccgagcaccaatgagctgcccttgtttgactttcctgcccaggaagtgtttgagttgcttggggtggaaaacgtgg
tgcagctgtttacgtgcgccctgctggaattccagatattgctctactcccaacattaccagaggttaatgacggtggcagagaccatcacaggcctgat
gttcccatttcagtggcagcatgtctacgtccccatcttgccagcctccctcctccatttcctggacgcgccggttccgtacctaatgggattgcactcc
aatgggctggatgacagatctaagctggagctgccacaagaggccaacctgtgcttcgtggacatagacaaccactttatagagctgcccgaggacctgc
cgcagttccccaacaagctggaattcatacaggaggtatcggagatccttttggccttcggcatcccccccgaggggaacctgcactgcagcgagagcat
ctccaagctgaggaacctcaaggcagccgacctggtgtccgacaagaggaacggaaacctggccggctccccattaaactcctatgaacttttaaaagag
aacgagactattgcccggcttcaggcgctggtgaagaggacgggggtcagtctggaaaaacaggtggaagtgcgggaggagtcgagcagcaaaaaggacg
gcaaagctcactgtgacgaggaggagctccgaatgcaccaactcaacatccaggtccgggaagtctttgccaaccgcttcactcagatgtttgccgatta
tgaggtgtttgtgatccagcccagccaggacaaggaatcctggttcactaacagggagcaaatgcagaattttgataaagcctcgttcctatcagaccag
ccggaaccctacctccccttcctctcccggttcctggagacacagatgtttgcttccttcatcgacaataaaatcatgtgccacgacgacgatgacaagg
atccagttttacgggtattcgattctcgggtggataaaattcgccagctaaacgtccggacccccactctccgcacatccatgtaccagaagtgcaccgc
catcgaggaggccgtaaaagccatagagcaaagactgtccaaaatagaccacacggcgatccacccccatctgctggatatgaagatagggcagggaaag
tacgagcctggattcttccccaaactgcagtcggatgttctttccacgggacctgccacaaataaatggaccaagaggaacgcgccagcacagtggagga
ggaaggacagacagaagcagcactcagagcaccttaggctggacaatgaccagagagagaaatacattcaagaagcaagaaatatgagcagcgggatccg
gcaacccaaactgtccaacctgtcaccctctgtaattgcgcagaccaactggaagtttgtggaggggctgctgaaggaatgtcggaacaagaccaagaga
atgctggtggagaagatgggaagggaagcggtagagctgggccacggagaagtgagcattacaggggtagaagagaatgctctgatcgccagtctgtgtg
acctgctggagagaatttggagccacggtctgcaagtgaagcaggggaagtcagccttgtggtcacacttactgcattaccaggacagcaggaggagagc
gacctggggcagtcttggcggaacagatttggtactggactgtgagagaaggaagtctgacccctttctggtcatgtaccccctaaagatctcacttgtt
caggatatgaggcatattcagaacattggagaaatcaagacagatgtagggaaggcgcgagcctgggtcaggctgtctatggaaaagaaacttctgtccc
ggcacctgaagcagctgctgttggatcatgatctcagcaagaagctgtacaagcgctacgccttcctgcgctgcgacgatgagaaggaacagttcctcta
tcatttgctctccttcaatgctgtggattatttctgctttactaacgtgtttacaactatcatgatcccctaccacatcctgattgtccctagcaagaag
ctcggcggctccatgttcacagccaacccctggatctgtatatctggggagctgggagagactggggtactgcaggttcccaggaatattctagagatga
cgtatgagtgccagaacctcgggaagctgacgactgtacagattggacacgacaattctggcctgtatgccaagtggctggtggaatatgttatggtccg
aaatgaaataactggacacacctacaagttcccttgtgggcgctggttgggaaaaggaatggatgatggcagtctggaacgtgtgctggttggagacctg
cttacatccagtacagacactgaagagaaaccatgccggacccctcccctgcagcactcacccagcatgatcaggaggcttgtggccatatcccccaata
gccgaccaaaattaaatacgggtcagatccaggaggctattggagaagcagtgaatggaattgtgaaacattttcataaaccagagaaagagagaggcag
tttaacgttattactgtgcggagagaacggactggtgtctgcccttgaacaagttttccagcatggaatccgatcccctcggcttttcaaaagcatcttc
atctgggacttcttagaaaaagcacaagcttattacgaatctctggaagtgaatgaattggtgccagaagaggactggcagaaacgggcgcggaatttct
cccgctttattaacgccattaacaacaaccctagaaacattgggaaagacggcaagtttcagatgtttgtttgcttaggagcccgggaccaccatttgca
ccactggattgcactgctggccgactgccccattatagcacaaatgtacgaggagacggctctaatcaaggaccacacgttggtgaactctctaatccga
gtgttacagacactacaagagtttaacatcaccttagaaagctccctcattaaaggaatcaatatctgaagcttcctgagaacacagcgagcgatacttc
acccccccagcagtgggcggcgacgtctctgtgggccccccaagtcccgccacaaagagaggggcttccgggagcgtcggaggattagcaagtcagatgg
agcgccatcccttgtgcgagcgactggttcaaggaaaggtccgaacctcttttctagtttgtttttcatacagaagtggtgaaagtgactattgcacagc
gtcatttccctgctgccatgtcggttgctttcgtccaatcacgtcgctctcatcccgtctccggaccacggtgaaagatttctccccgtttaaatatctg
ttccattgtatagatatttgctttgaaggcatgtcatcacttatttgctgtcacagaaatatatctctatttttccatccgatcgtgctagatcccggtg
ttcgtgcagaatttcccttgtcagtattactgtctgatcggaggggcgcagctattgtagatcacgctacgcagacacacagtgatttttttttttcacc
ccccctcggctttatttaactcattgtgtgtttgttattctgtctttctgtggaacaaagatgtgtaaccagcgactacaactcccgggaccatgggtgg
tgctgggagttagccttttacagcagttagtgggttgctggctggatatctaaacaggggtgtgtggtaatacaggggcaattggtggtttctttattgg
acactccccttcctcggagtcttttctaaactgcccaatgtcatttgcttcttattcgcctgtgactccctcatcctgctgcttctctggccgcctacca
tgcaataactaatgttaggtgatggcacccataggaactgttccagaatgaccggacctctgtcacgccattttctaactgtgctagactttaaacaaat
aaacctattctcacggttggtcatctggatcagaaccgagaggcacttttgcacagagcaggctaaaaataaatatatttacacagtaaaaaaaaaaaaa
aaaaa
5' EST: CX923948 [+]caggacagctgtccggtgggctcccgtgatgcagcgcgcagagccgtgtagccccgggcccccggaggaggatgacaggcgggagaggagggcggtttga
cagcagcaccatgagcgggggcgggggaggcagcagcacggccccgggccgtttcgccgattatttcgtgatctgtgggctggatacgggcaccgggctg
gagccggacgagttgtctgcattgtgtgagtacatacaggcttctaaagccagagacggggctggacgtttcttacccagtgcggctgaaggagagaatt
ttgagcagacccctctgcgccgcacctttaaatccaaggtcttggcgcggtacccagagaacgtggagtggaacgccttcgaccaggatgccgtgggcat
gctatgcatgcctaaaggattagcatttaagactcaggccgaccccaaggagccccagtaccacgcgttcatcatcacgagagaagacggctctagaaca
tttgggttctccctcaccttctttgaagaagtcaccagcaagcagatatgcagcgccatgcagaccctgtaccacatgcataacgccgagtatgacatca
tccacacgtcggcgagcaacggcagagactgccccggagagtgcaccgacagctccatcaccaagctgcagcgcttcaactcttatgacataacacgaga
caccctgtatgtgtccaagtgcatctgtctcatcgcccccatgtctttcatgaccgcctgtaagaaagtcctggagcagctccaccaggctgtgacttcn
ccacagccgccgccatt
3' EST: CX923947 [+]actgtgtaaatatatttatttttagcctgctctgtgcaaaagtgcctctcggttctgatccagatgaccaaccgtgagaataggtttatttgtttaaagt
ctagcacagttagaaaatggcgtgacagaggtccggtcattctggaacagttcctatgggtgccatcacctaacattagttattgcatggtaggcggcca
gagaagcagcaggatgagggagtcacaggcgaataagaagcaaatgacattgggcagtttagaaaagactccgaggaaggggagtgtccaataaagaaac
caccaattgcccctgtattaccacacacccctgtttagatatccagccagcaacccactaactgctgtaaaaggctaactcccagcaccacccatggtcc
cgggagttgtagtcgctggttacacatctttgttccacagaaagacagaataacaaacacacaatgagttaaataaagccgagggggggtgaaaaaaaaa
aatcactgtgtgtctgcgtagcgtgatctacaatagctgcgcccctccgatcagacagtaatactgacaagggaaattctgcacgaacaccgggatctag
cacgatcggatggaaaaatagagatatatttctgtgacagcaaataagtgatgacatgccttcaaagcaaatatctatacaatggaacagatatttaaac
ggggagaaatctttcaccgtggtccggagacgggatgagagcgacgtgattggacgaaagcaaccgacatggcagcagggaaatgacgctgtgcaatagt
cactt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
