XB-CLONE-3639848
|
Clone Name: IMAGE:7660668
|
Gene:
phc1
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX841342 
[+]
agcagacagagagtgagggagacatctcggagtgagcggcggggacggggcagagggcaacgcgggagtccggggcagagggaactgaagctctgcatgg
attgaatcatttttctatcaaacttctcaccttggtgaaggtcccactatggaaacagagagtgaacagaactccagctcaaccaatgggagctcagcgt
cggggagcagtacgcgccctcagatatcccagatgtccctgtatgaacggcaagctgtacaggccctgcaagctctccacagacagcccaatgctgctca
gtattttcaccagttgatgctacaacagcagctgagtaatgcgcaactacacagcctggcagccgttcagcaagcaaccatagctgccagtcggcaggcc
gcatcccctaacacaggctccgcccagcagacgaacaccacccagggatcgcaggctaacctttccacaagctcttcagcccagctcctgagccgtaccc
agaatgtcaccgcccccagcgccactaccctcagacagtcagtgttgcttagtaatgccagctcgccgcctcttggccagtcacagacacagatgtacct
gcgccagccacagctagggaaccttctacaggtcaaccggtctcttgggcgtggggttccactgaactcgcagctcattttaatgcccaatgggtcggta
gctgtccagcaggaggtgacatctccccagaaccaaggagtgcatcaggactcggatcaagttcagaatcttgctgttcggggccaacagactgcggcaa
ctacttcagcagcaggagctacaatctcccagccagctcctctggctaccaaaccccaaatccacccag
3' EST: CX841341 [+]aagggtgtgaacatgactttattatctgtttaattaagatgtttacacaaccagaagtggagcagccgacagggccgagtacaaagggagtatccccaaa
atacttagtgaattgtgatgtaaagaactggaacccataggagtaaacctggttctgcagaggtctcagtgcactttggctcagcccgtgtatataaaga
gtgctagttaaaggcaaaggaatgggagatgagccaatgggaataactgatataaaaagcaggaaatgtaatgcttccttataggccgggccgggagtag
cctcattctccatgggtataaagacaaatcagatgggaattgttgagcacaaggagtgtgaggttgggaattacatctgttctaacatgggggggggggg
gcagcaatatttctttattgcccccctgagcaggggtgactgtatgccccatagccattttgatctgtgtcgtaatagtcgtaagcataaaccgagctgg
gcgccacattcagcacccggttacccatctccatcttcaaagggagctcaactgtcttgctggaaacagagcttaaatacaggaaaacatgattgttctt
cagttcatatctggaaatcagtttttcattctccagcttagggtgacg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
