XB-CLONE-3646150
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Clone Name: IMAGE:7657139
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Gene:
zbtb20
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123955 
[+]
GGGAAAATGGAATCATTAGACAGGCAGCCAGTCATTACCACTTACACTCTAGTTCCAGGGAGACTTCGATTATGAGAAGAGAAAACTCTCAGCTCTGGGG
GACAAGGATTAAAGATTTCATAGCTGATTTCATTGAGTCTGCGTGAAGAGCCAGTGCTAGGAATTGGCCCCGCTCCTCTCTCGGATCTGAAGGCAAGACT
GCCGGGAGTCCCACTTCCATGCTGAAATCCCGGCACCTTAACAAAACAGAGAGATAAAGCTGCGCGGCGTCGGCGCTGGTTCCGGCCCAGGTGCTGCACT
ACACAAGACCCAATAAGGACGGGATACGTCGTGTCAATGACTGGGCCCCAGCTTAGCCATGGTTTGGTGGATCCCACCGGGGCCCTCGAGTCACAGTGAG
TGCCGGCTGCCGTAGCAGGGACCCCCAGCGTGCCCTATGTGGATGATACCGGGCGGAGCGAGGAACCCCCGCCGTGCTCCCCAGCACCCAGACCTGCTGC
ATTAAAGAGCTTCGATGCTGCTTCTGTTCTCTCCCTTCCCAAATGATTGTCTGCACTAACCCTGACACTTGGAGTGACCTGCCCGAGGAGTGACGCCACT
GAACTACTGAAGAAACGCAGGACAGCTGAAAAGCAGAAGGCGTCTGTGGAGAATGAGATTAGTCAGTGGAGAGGAGGCAGTGCCAAGCCAGGCCGTCCCT
GCCAGAACTTTCTAGCTGTTCTATTCCGCTGCCCAGCCTTATCCAACTCATACAGACATCAAGCAACCAATCAGCACGCGTCGCCTGATAGTGACATCAG
TTGCAAGGGGATGACAGAGCGCATTCACAGCATCAACCTCCACAACTTCAGCAATTCCGTGCTCGAGACCCTCAACGAGCAGCGCAACCGTGGCCACTTC
TGTGACGTGACGGTGCGAATCCACGGAAGCATGTTGCGGGCCCACCGCTGCGTGCTGGCCGCTGGCAGCCCATTTTTCCAGGACAAGCTGCTGCTGGGAT
ACAGCGACATCGAGATTCCCTCGGTGGTGTCGGTTCACGCGGTGCAGAAGCTGATCGACTTCATGTACAGCGGGGTGCTGAGGGTTTCCCAGTCGGAGGC
GCTGCAGATCCTGACGGCCGCTAGTATTCTGCAGATAAAGACGGTCATCGACGAGTGCACGCGAATCGTCTCGCAAAATGTCGGAGGAGTGTACCCCCTT
TTACCGGACTCTGCTCAAGAGACGCCCAGGGGGACCCCCGAGTCGGGCACCTCTGGGCAGAGTAGCGACACCGAATCAAACTACCTTCAGAGCCATCGTC
AGCACAGCGTTGACCGGATCTACTCCGCTCTGTATACGTGTTCCATGCACAACGGCAGCGGGGAGCGGTCCTTCTACAGTGGAGCCCTGGTTAGCCACCA
CAAGACTGCCTTAGGGCTGCCTCGGCAACACCACATGGAGGAACCCAGCTGGATCACCCGCATACACCAACGATCCCAGAGAATGGAGCGGTACCTCTCC
GCCACTCCCGAGACCACCCACTGCCGGAAGCAGCCACGCCCGGTCCGTATCCAAACACTAATGGGCAACATTCACATTAAGCAGGAGGTGGAGGACGACT
ATGACTACTACGGGCAGCAGAGCTCGCAGGCGCTGGAAAGGAACGAGTCGGAAGAGTGCACTGAAGACACCGATCAAGCAGAAGGCACTGAAAGTGAACC
CAAAGGCGAAAGCTTTGACTCCGGAGTCAGCTCATCCATCGGCACAGAGCCCGACTCCGGAGAGCAGCAGTACATGCCCATCCTCAGCCGAGAGGGTCCG
CAGGAGCCTTCCCAAGCAGAGTCCAACAACCTGCCCACCGAGGGCAGTGAAAACCAGGACCAACCGCACTTAGAAACCAGCTCTTCCTCCCCCGAGAGGA
ACCTTGATACGGACAGCACAGTGCTCCGAGTTAGCAATAGCACCGACAAGGGGGTCGTACAGACTTCGGTTACCCAGTCCATAGCGCAGACAATGCCAAA
TACCCAACTCTACTTACGTCAGACAGAAACTCTCACCAGCAACCTGAGGATGCCACTCACCCTGACTAGTAATACCCAGGTCATTGGCACCGCTGGCAAC
ACCTACCTTCCTACCCTTTTTACCACCCAAGCAGCCGGGAGTGGCCCCAAGCCTTTCCTTTTCAGTCTGCCCCAGTCTATACCTGGTCAGCAAACCCAGT
TTGTGACGGTCCCCCAGTCTGGCATCTCTCCATTCGCTGGCCAGTATCAGAACACTTCCCAAACCAGTCACAGCACATCAAGTGCACAAGGAGAAAAAAA
GCCTTACGAGTGCACCCTGTGTAACAAAACTTTTACCGCCAAACAGAATTACGTGAAGCACATGTTTGTACATACAGGGGAGAAGCCGCACCAGTGCAGC
ATCTGTTGGCGCTCCTTCTCCTTGAAGGATTACTTAATCAAACACATGGTAACGCACACCGGTGTGAGGGCCTACCAGTGCAGCATCTGCAACAAGCGCT
TCACGCAGAAGAGCTCCCTCAACGTCCACATGCGCCTTCACCGGGGGGAGAAGTCCTACGAGTGTTACATCTGCAAGAAGAAGTTCTCCCACAAAACCCT
GCTAGAAAGGCACGTGGCCCTCCACAGCGCTACCAACGGCACTGCCAGCCTCCCAATCACCGCTGCACCCCCAGGCTCAGTTCCAGCCCCAGGTTCAGCT
CAGCCTCTGGGCTCGGCTCCAATCTCTGGTACCACTCCCGGCCAGGGATTGCCACCGACCCCCAACATGGCTCCAACCCCTGTCTTGGCCAACGCTCAAG
TCTTGCCTGCGGCTCCAGTCTTAGCACCGGCTTCCGCCTTGGTACAAGCTCCAGTCTTGGTCTCTGCCTTAGCACCAACCCCAAACCTGGTTCACCCCCC
CACGCCTTCCCCGACCCCCGGCCCCGTTCTAGCCCCGCGGCCTCCCAATGGCGGTGTCGCGACCGGTGCCGAGGGCACAACGTACGTTTGCTCAGTCTGT
CCAACTAAGTTTGACCAAATCGAGCAGTTTAACGACCACATGAGAATGCACGTTTCTGATGGATAAATATCGTCCATTCTTACGAACAAACAAAAAAAGC
TATGGCACTAGAAATTTAAGGTTTTTTGTTTTTATTTTATTTTTATTTTTTTTGGCATTTTCTACTACATGAAGAACTGTATTGCCTGCTGGTACATTAC
ATTTCCGGAGAGTGGGGTTAATATATATATCTCTATATATCAAGGTATATAACCTGCGTATATATATGACGATCCAGTCTCCCTCAGATGGTGGCCTTAA
GGCTTGGTAGATTTACCCGAATTCTACTGGTTGGAATTCAAGCTACTGGCCTCTAAATACAACCCTTCTTTAAAAAAAAAAACAACAAAAAAAAAAATAA
CAAAATCCACAAAAAAAAAAAAAAA
5' EST: CX828352 [+]ggaaaatggaatcattagacaggcagccagtcattaccacttacactctagttccagggagacttcgattatgagaagagaaaactctcagctctggggg
acaaggattaaagatttcatagctgatttcattgagtctgcgtgaagagccagtgctaggaattggccccgctcctctctcggatctgaaggcaagactg
ccgggagtcccacttccatgctgaaatcccggcaccttaacaaaacagagagataaagctgcgcggcgtcggcgctggttccggcccaggtgctgcacta
cacaagacccaataaggacgggatacgtcgtgtcaatgactgggccccagcttagccatggtttggtggatcccaccggggccctcgagtcacagtgagt
gccggctgccgtagcagggacccccagcgtgccctatgtggatgataccgggcggagcgaggaacccccgccgtgctccccagcacccagacctgctgca
ttaaagagcttcgatgctgcttctgttctctcccttcccaaatgattgtctgcactaaccctgacacttggagtgacctgcccgaggagtgacgccactg
aactactgaagaaacgcaggacagctgaaaagcagaaggcgtctgtggagaatgagattagtcagtggagaggaggcagtgccaagccaggccgtccctg
ccagaactttctagctgttctattccgctgcccagccttatccaactcatacagacatcaagcaaccaatcagcacgcgtcgcctgatagtgacatcagt
tgcaaggggatgacagagcgcattcacagcatcaacctccacaacttcagcaattccgtgctcgagaccctcaacgagcagcgcaac
3' EST: CX828351 [+]gtggattttgttatttttttttttgttgtttttttttttaaagaagggttgtatttagaggccagtagcttgaattccaaccagtagaattcgggtaaat
ctaccaagccttaaggccaccatctgagggagactggatcgtcatatatatacgcaggttatataccttgatatatagagatatatatattaaccccact
ctccggaaatgtaatgtaccagcaggcaatacagttcttcatgtagtagaaaatgccaaaaaaaataaaaataaaataaaaacaaaaaaccttaaatttc
tagtgccatagctttttttgtttgttcgtaagaatggacgatatttatccatcagaaacgtgcattctcatgtggtcgttaaactgctcgatttggtcaa
acttagttggacagactgagcaaacgtacgttgtgccctcggcaccggtcgcgacaccgccattgggaggccgcggggctagaacggggccgggggtcgg
ggaaggcgtgggggggtgaaccaggtttggggttggtgctaaggcagagaccaagactggagcttgtaccaaggcggaagccggtgctaagactggagcc
gcaggcaagacttgagcgttggccaagacaggggttggagccatgttgggggtcggtggcaatccctggccgggagtggtaccagagattggagccgagc
ccagaggctgagctgaacctggggctggaactgagcctgggggtgcagcggtgattgggaggctggcagtgccgttggtagcgctgtggagggc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
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