XB-CLONE-3663593
|
Clone Name: IMAGE:7646631
|
Gene:
pdxk
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX804296 
[+]
cacagtcattccccgcccccgcccgtcactcacagacggtcccgccccaggcccctccccccggtcagattagctgccccggctgtgcctggctgggtat
ctccgtgatctgcccccatggagctgcccgggagccccgagtgccgggtcttttccatccagagtcacgtggtgcgggggtatgtgggcaacaaggccgc
ctccttccccctgcaggttctggggtttgaagttgatacagtaaactctgtgcagttctctaatcatacaggttacaaccactggaaggggcaggtgctg
aatgccgaggagctgcaggaactctacgagggcctgaaactgaatggagtcactcgctacgactatgtgctgactgggtacaacagagacgcctccttcc
tggctcgggttgtggatatcatccaggaactcaaacggcaaaaccctcatcttgtatatgtttgtgatccagttctgggagacaaatggaacggagaggg
atccatgtacgttccggaggaactgctcccggtatatagagatctggtggtcccggtcgccaacatcatcacccccaatcagtttgaggctgagctgctg
accggcctgaagattcgcacaaagatggaggcagtgcaggtgatggataagttgcactcgcttgggcctgacactgtggttatcaccagctcggaactcc
cagcatcccgtggggcagactatcttgtgacgctggggagccagaggaaggtggacgcacaggggcgaatccacacgcagagaatcagcctggagttgcc
ccgcgtggaggccgtgttc
3' EST: CX804295 [+]agcagatcacagataatttattagacgctctgagagccaattatttccatctgatcacagttagtagaataaaataaaaatttaccccgtggggttgaag
cacatggaacaaacagggcccgttagcagcgttggactagtagtagccacagggttgggaggcgggtgtcagtacgggccgctctatacagtaacatggg
gaatgggctccaggtacagtcccatatgtgccccaggggcagctcaattgccccccagtaccactaaaacctgcacgtagcagctacagatcccccgctg
tgtgtggcatcagcctaaatcctggggggtatgtggggtgttcactctcccttggcaccacccacccgtgatgcagcccaaccagttctatgccaggatc
cagtatggccgccatcgggcccagtgcccccccagaaagtgctgaaggttcgaacattatcattgggtttagtgttcccattgtgaccaaactgaaacag
gccggcccaataagcagctgtggcaacgagccctctaacagtgcttgccccactgtgtctcgggggccctaacgattgggtgaatggggggggagttaga
atgcgtgctgatacagcaatatctgcccccaggctggaggtaccggggtgggtcggaccccaaacctctgccacaaggtatttcacttacaccagtgccc
atctgcat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
