XB-CLONE-3686386
Clone Name: IMAGE:7513876
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Gene:
eif3i
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX743536
[+]
cggaaacagttggctctcttagagacaaactcagctgtaaggacctgcggcttcgattttggtggcaacataatcatgttttccactgacaaacagatgg
ggtaccagtgctttgtgagcttcattgacttgagagaccctacacaaattgaagacaacgagccatacatgaaaattccttgcagtgagtcaaagataac
cagtgcagtgtggggaccacttggagagaacataatcgctggccatgagaatggggagctgaaccagtacagcgccaagtctggggaaatagttaacagc
atcaaagagcattccaagcagatcaatgacattcagacatcccgagacatgacaatgtttgttacagcatccaaagactgcacctccaagctgtttgaca
gtacatcacttgagcatcagaaaactttcaggactgaaagacctgttaactctgctgccatctcacccatatatgaccatgtagttttaggaggaggaca
ggaagctatggatgtaaccacaacatcaacaagaattggaaagtttgaggccaggttcttccatgtggcttttgaggaggagtttggcagagtaaaaggc
cattttgggcctattaacagtttggcattccatccagatggcaagagttacagcagcggaggagaggatggatacgttagaatacattactttgacccac
aatatttcgactttgaatttgagtcgtgagacagctgcttcgtgcttggttatatcctacttcacatctntgcatacacacacacacacacacacacaca
cacacaattgtattgactgctcaatgatatctttacaataaatgttactatatttattgaagcaaaataaaaatactttgccactgttctttcc
3' EST: CX743535
[+]gagttaaggcagtttatttctcatactgtcagatgttgtttcagagggaaaatacaaaccacccctgacatcaaagcataaatccatttaatagaaattc
atgcctgttgctttgtcctggattaatgataattgcagtatgtttccaacctgagaagctgcatgtgaattaaaggatgtaattactgagccctctctgt
atagcacacccaatgattaaagtgatactgacactaaaaaactactctcaaatattaaagtacagtaaaagttacctatagttctagttgatagtctgct
tttctaagcaatggttacttgaagatcctcaatatgactgttttaccaacctgtcagagttttaaatgctaaaagactactgcagccccctcacagagga
aaaagggggatgacataggtaatgtaaaagcattggccaaatacttttaaggcaaaattataaatagcatgcaaaaaaagtgttacagtagatttcttgt
gtcactatctcttaaaaatgcattgtgttattttccttcccacagataaatgtatgtgaaacattttggtggggaaagaacagtggcaaagtatgtttat
tttgcttcaataaatatagtaacatttattgtaaagatatcattgagcagtcaatacaattgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtatgcaaaga
tgttgagtaggatataaccaagcacgaagcagctgtctcacgact
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGCTCT(20)V-3'). After ligation of PstI adapters and XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Vector Details | Vector Map |
Name: pCS22+
Type: plasmid
5' Restriction Site: PstI
3' Restriction Site: XhoI |  |
Usage:
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