XB-CLONE-3915419
Clone Name: IMAGE:7091987
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Gene:
arrdc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654527 [+]
taattaatcccccccccccccggtctttgtaaagagaccatagaagagagcactgtgacagcagctgctgctgttacttctcctacctgttcttcccgat
gagctggccgctgggcgagcactgagcctgcctgcagtctctgcacggcaaatgcatactccagtgctctggcaacatcgccattgatgtgaaatcggaa
acttttgtctcccccccagaatatttttatagcccccttttacaatcatggtgctggggaaggtgaagagtttgaccataagctttgactgcctcaatgg
cagccacgtccccgtctattccagcggggatgcagtctcaggaagggtcaatctggaagtgacgggggaaattcgggtgaaatccctgaggatccatgcc
agggggcatgcaaaagtcagatggacggaatcaaggaacgctggctccaacactgcctatacccagaattatactgaagaagtggagtatttcagtcata
aagacatcctgattgggcatgagagagatgacgataattcagaagaaggactaaccactattcattcaggaaggcatgaatatgcatttagctttgagct
tcccaaataccactttgctacctctttgaaggcagactggcagtgtgcgctctgggtgaagcggaatgcacgaccctgcttctacgtaaaataagaagaa
tcccagctttgaaactagattcacc
3' EST: CN327766 [+]ctttgttactactaggatgtgcatcctcatctcttgaccacatacgaatgcagcagcttctcgactcggcccacaagacggtgggagagcaaccgtatgt
atacagcacacacaccttataatgcagtatatgctgtaaccatgaccagaggctgattaagtctgccacttccaaatcgaaaaagcagtttcctttgaaa
gtgcgcctccaacataactgccataacttaacaggcagttaagagtcttgtctgtaaggaaaaagaatccctcagcgagatgggcaggaaggatgctctt
ctgtaggctgatctggattaggatcaacctctgaatatagtggggggggaagaaaacgaaattcttggatgtatgcaaatagtggtccttgaagagccaa
ttcaaaattgtcacaagcatttgaagacatcagattctgcctttgctcttctgtgacaacttctgcatagctgggaggcgcttcaggtctttctggcaat
gtcaaaccaagccaattatttatgctgtactggctgctcacactggatgtccggctgccaaatggatgcagtgggatagtgccaattaccaatggtaaat
taaggaataaatccatagcccctggaatatccacatataccataagagaatattccacacggattatgctacagtcaatgatagaaagggaaatgggtgg
aattttcagcagcttgccattccaagtctctgtttttcctgacgacaagggactctctccccagttgggaacaagttgcctttcttcccttcttttttcc
tttgggcaaaaaatgttctgaattttgataaaaggggcggccttttgggaccccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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