XB-CLONE-3916487
Clone Name: IMAGE:7091821
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Gene:
ppib
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654351
[+]
taattaatcccccccccccccgctgttcatgctgcggccgctggagagaaacatgaagctgctggttgctgctgcgctcatcgcgggctccgtgatcttt
ttgctgttccctgggagctccgtggctgatgaaaaaaagaaaggaccgaaggtgacccataaggtgtactttgatatcaagattggtgatgaggatgtag
gacgtgtagtaattggtctttttggaaaaactgttcctaagacagttgagaactttgtaaccttggcaacaggagagaaaggctttggttacaaaggcag
caagttccaccgtgtgatcaaagacttcatgatccaaggaggagatttcactcggggagatggtactggaggaaaaagcatatatggagaccggttccca
gatgaaaacttcaagctaaggcactatggtccaaattgggtgagcatggctaatgctggcaaagacactaatggttcccagttcttcattacaacagtca
aaactccatggcttgacgggaaacatgtagtgttccgaaaaattctggaaggcaaggatgttgtcgaaaagattgaatctactaaaacagatggtcggga
taaccaacttaagatgtggtgattgcagactggggaacattgaagtggaaagccctttgcaattgccaagaataatgtaaattgagggaagacccgttgc
ctcttgcca
3' EST: CN321251
[+]tttttttttttttttaatagaaatggtttttattcaacccaacaattattgtacatgtccatttcaaatttgagtacaaatttttagtggatggaatgta
taattcaattatttgttcttgatcctaagaacaaaaaatggatttaccgtctggccactcataactttaataactgaaattaaaagttcaggggtataaa
gacagacctaaatatgtgcctgaaacttccggacatgggtgtttttccccatgccttgttagtgaggaatgaaaagggtacacagcatgtaactcacagg
aaaatcctcaaaactgatttaccccatgatggacaaataagcaacagggtccttccctccattttacattactccttggcaattgccaatggcttttcca
cctcaatccttccacaatccgcaatcaccacatctttaaatggtttatccccaccatccgttttaataaaatccatcctttccaacacctccctgccctc
caaaatttttcccaacaccacctgtttcccctccagccctggaattttgacaggtgtaatgaagaaacgggaaacattaatggctttgcccaccttaacc
ctgccctccccatttggaacctaatggcctaacctggaattttccttcgggaacccggcctctatattgctttttcctccagtaccacctccccaatgaa
atctcctcttggatcatgaangtcttgatcacacggtgggactttgctgcctttgtaccaaagcctttctctcctgtttgccagggtacaaaagttctca
actggtcctaagaaacagtttttcccaaaaaaaaccattttactacccct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI |  |
Usage:
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