XB-CLONE-3916595
Clone Name: IMAGE:7091331
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Gene:
azin2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653607
[+]
taattaatccccccccccccccgagcgcatacactgtacacagagctggtgcggtttctaggcttagttccacatttacattaatgggcaacggccgtgt
caatgcatgttaagaaagcgatagacgccagcagcggcgacaacatccacagcccggggtgctgcaaaatctccgagtttactgctgagcccccctggtt
cctctgataaaagcttgttgcatacttaaaaacaatcttggtaaaaaggcttaaaatcccagatttcacaatgcaaggatatatccaggagtcagatttt
accttggttgaagaaggctttatggccagagatttgatggaggagatcattaatgaagtctcacagactgaggatcgcgatgccttttttgtggctgatc
ttggggatgtcgtaaagaaacacctccgttttctgaaagctttgcctcgtgtgaagcctttctatgcagtgaagtgtaacagcagcaggggagtggtgaa
gatcttggctgagctgggagctggatttgactgtgccagcaagacagagattgagctggtccaggacattggtgtgacaccagaacgaattatctatgcc
aacccatgcaagcagatttcccagattaagtttgcagctaaaactggtgtccagatgatgacatttgacaatgaggtagagctgtccaaggtgtcag
3' EST: CN320642
[+]tatactccatcactaacataatacataatgttcttattgggcccattttcctcatcatcagacccagaatgctccattagcacttctttcttagcaataa
tgttaacagccaatgaaaaagcagatgctacatagtatcttcctggttcagcaataatttgcacatcagagccctcagggaaatacaggtctagtgctgg
atttataacacccgccatctcttcaaacctaatcttggaatcctctgttccagggaagccacctccaatgtccagcagccacatattgtacccaaactcg
gatgccatttcaaaaaccaagcgtgcatcagcaatagcctgagtataggccttggaatctgtgcatccactgccaacatggaaactaacaccaatgacat
ccacactgaggtttttagccatttccagtaactgtctgcaggattttaggggggcaccaaactttacacttaaacgagcagaagatttagagtcgtctgt
agctatgcgaagtaccatcctggcatttggatggcttcttgacaccttggacagctctacctcattgtcaaatgtcatcatctggacaccagttttagct
gcaaacttaatctgggaaatctgcttgcatgggttggcatagataattcgttctggtgtcacaccaatgtcctggaccagctcaatctctgtcttgctgg
cacagtcaaatccagctcccagctcagccaagatctttcacactccccctgctgctgttacacttcactngcatagaagggcttccaacgaagcaaagct
ttttnaaaaaacggaagtggtttccttttcaaacatcccccaaaatcaaccac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: