XB-CLONE-3916837
Clone Name: IMAGE:7092066
|
Gene:
ctnnd1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CK654590 [+]
taattaatcccccccccccccggatactcccgggatgtagcgtcacagcttggggtctctcttctctatatggatgagccagagtctgaaagtccggcca
gtattttggcctcagtgagagctcaggaggcacagtttgagctgctgtctcgtgctctggaagaggaaaggagacacatcactgctcagctggaccgggt
gtgggtcactccacaggagccacccctgaccaatggctcccgcacccgttgccagcaggacgggtcaccatttttgtacacctcaactaggatggaggcc
cactctgttcatgcagatgagagatatgtcattgatgatgattcatcttacaaatcatgcactctaaacgatgaaactcgatgcaatgaaatcccagtac
aaacaattgtgggatactctcaaacattagatcgaccatacagggagcctgctggaggtggaggaacttatactacagtaccaagaaattaccatttccg
cggtcctggagtcgattcaacaatgccagtgatttcccagtctccacatcctggttatagtagcctatcaaggccaaatcagcgatatcgtagtattgat
ccttaccgaagtcctggatatggacccaaccacaggttagggcggtggtcttgaagcaccagtctgatt
3' EST: CN319295 [+]attctgagagttcttcagagcggtttgggaggcgattgctactatcaccattatgagcgaacggatctgttctgaaatcatttagcggaatgtcagtatt
ctcagattttttctcattttttaggtttcggtcaatgagtggaagagtactatcatcataaacctgcacacgggaagttaccgcagtggacacctgaaaa
tcatttcttttccacccatctttttccagtggtcttctcagttctttataaccccaaatggtctgaaggcaaaaaccagcagcccgtatctctttgtctg
aacgacctccccctttattaataaggacaagtctttcaattccctgactctcacgcaatctttttgctgcctctagattcccagaaataacttcatgaat
agtactgataacgccgacattcgtatcttcagaaagtgcagatgactgggcagaggaactagacagacgtgcaacaagactgttcagagcatgttttccg
atcagttccctgttcctgctgtctccacaaaggttgcgcaatgctccacaaatggcacgaacaacacgctcactctcatgggttaagtgatcagcaagtg
aagaacgacctttctcctgttgaactggcaccccaatgccgcggccttaaacccaatttccagcccacagggttttgaattgtcccccctgaaacctcta
attccacaggggtgtgtgaaaactttacaaaaaaaaattaaaggccccctatctcctgggtggaaaaaaaaggactaaccctttggcttgggcttttttt
tttttgggaaaacaaaatttggtaaaaaaaaaaatcttctttaaaaaacctttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: