Summary Attributions

Clone Name: xthr04D22	Gene: ckm , atp5mf	Species: Xenopus tropicalis

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Sequence:

5' EST: CN120501 [+]

gctgtgacatctcggtcattaagatggcggacaagccggtgcccctagctgagagaagactcatggatattaagcttggtcagctgcccaactggctgtc
aactcgtgatttcacaccaaatggaatcattgcatcattacgcaaaggacataactcctatttcaacaagtacatcaatgtgaagaagggaggcattgga
ggagtggccatgctcctggctggttatgtagttctgagctatgtttgggaatatgatcatatcaagcatgatcgctggagaaagtatcactgatctgagg
aaatcattccttcttctatacttaatgtttcctgtacagtttcaagccagtccttgataaataaatataaaaagtggattac

5' EST: CN117931 [+]

ggggtcaccctgaagtttgctccgtctctaaagcaggtctagcccgatcttccaacatgcctttcggtaacacccataataactggaaattgaattattc
ctctgaggaggaatatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacacca
agtggcttcaccttggatgatgtcattcagactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcct
atgaggtgttcaaggacctttttgatcccattattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaacct
taagggaggtgatgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgt
ggggagcgtcgtgccattgaaaaacactccatcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatg
cagagcagcagcagctgattgatgaccacttcctgtttgac

3' EST: CN117930 [+]

ggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatcagagaattagatttatcttctcttttatgttgattttgaagttt
ttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcccctgtagatggccattttttgacctct
aatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcccctgtcatctacttctgtgctggcatc
atatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactaccatctggacttggtccacttcagagaagc
ccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctc
atcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagtttgatgggcaggtaaggacataacccaga
tgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcacagaagcgtctgaagacttctttcatgtttcctc
ctttctgcatggaaatgactctcagatggtcctct

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Library:

Name: Xenopus tropicalis xthr plasmid library

External Dbs: Unigene tropicalis cDNA library

Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).

Anatomy: head	Stage: NF stage 25 to NF stage 35 and 36
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: SfiI 3' Restriction Site: SfiI

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Usage:

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Suppliers:

Search for clone at: Open Biosystems