XB-CLONE-3918464
Clone Name: xthr04N09
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Gene:
ddx54
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN120466 [+]
gaggtactgcagggagagaagaaagaagctggacattaacccgacccactgacaggatgaagtgctttgtggtcctgctggtttgcatctccattgggtg
ggtccattcctgcaaattcaggcaggaagaaagtaatctaataaagggagcaactgccaaacaaagctccacttctagacctgattactcggctgaaaga
gcctttgatggtatcagtgattacaacatgatgagacacccttgtgcagaaactaggagagactattccccttggtggcagttggacctgaaacataatt
acaaggttgattcggtggtcatagtgagccggagggactgctgctttcatacactgttgggagctcagattcgcattggaaattccccagataataacaa
cccagtgtgcggcaccatcaccgacgtcttcagacccatcaatctgtgctgtaatgggatggagggtcgctatgtgagtgtggttatccccgggcgggtg
gaacagctcacaatctgtgaagtgaaggtttatggtgaaggtgccaatagaaagttatcccaagatgaac
5' EST: CN117871 [+]agatgtggctgctcgaggtattgacataccaatgttagacaacgtcataaactacaacttccctccgaaagccaagcttttcctgcatcgtgttgggcgt
gtggcaagagcagggcgaagtgggactgcttactctcttattgctcctgatgaaattccctatgtgtatgatctccatctctttcttgggcggccgctta
aactggcaacagcacaaatgatggagacaggagcagacacagatggggtactggggcgagtaccacagagtctgattgatgatgaagatgccttgcttat
tacggatcgtgagcgatcgctggagctgcagaacttgcacaacgtggcggagaatgcctacaaacagtacattaaatccagaccaactccctctcccgag
tccatcaaacgagtcaaggatgctaactataacctgttgggggttcacccactcttttggtctcagcttggtgaagaagagatgcagaggctgaagtttg
tggatggcatcaaatcttacaaatctaaagcaaccatatttgaaattaacgctactaataaaaccaatgcaagtgaaattatgcgagccaagagaagtca
tgatgggaaggtcatcgctaagttccagcgcctgcaggaggagaaaagtgcaac
3' EST: CN117870 [+]tttttttctttcatccacctcatgacttgtttgttcttcctgagatcttccgtctggtctcccaataggtccaacacagcccctgcagctgcctgctcaa
agctgctggccccaatgctgagccccctctcactctcaaaatcctttggtctgtatgggatgtaatagtcagtgtcatgagaagttcctgctttcctttt
cttgttgccttcagcttcatctttgtcagtaattttcctttttccaagcacttgcgaaaatactccctccatgttctcctcatcactctcttgtttgcag
ctaaggctggagatgccactggtcaaagtctgcactgttgcacttttctcctcctgcaggcgctggaacttagcgatgaccttcccatcatgacttctct
tggctcgcataacttcacttgcattggttttattagtaacgttaatttcaaatatggttgctttagatttgtaagatttgatgccatccacaaacttcag
cctctgcatctcttcttcaccaagctgagaccaaaagagtgggtgaacccccaacaggttatagttagcatccttgactcgtttgatggactcgggagag
ggagttggtctggatttaatgtactgtttgtaggcattctccgccacgttgtgcaagttctgcagctccagcgatcgctcacgatccgtaataagcaagg
catcttcatcat
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: