Summary Attributions

Clone Name: fgf10-HAR400	Gene: fgf10	Species: Xenopus tropicalis
Description: EXRC Clone Number HAR-400. Xenopus tropicalis fibroblast growth factor 10. To generate probe for in situ and mRNA for injection. Clone previously called: TNeu116h04

---

Sequence:

Full length sequence: CR761890 [+]

ACACAGTCAGCTGCGCAGCTGGCAGAAGACGCAGAAGATTTAGAAGCAGCAGGATCTGGAGCACTTGGAGATACCCAGGACTTTGCTATGGACATAAGAG
GTCCCTGAAGCTGGTTTGCCAATCGAGTTATGGATGTTGGTGCCTTGTTCTCTGCACATAGGAAAGTCCAGGGTCCCGTTGGGCAGCAGCTCCCCAGTTG
CCATCTACACGCTCTCATCCTTCCAGTATGTTTCTTTCCATGAGGCATTTGCTGATGCCGACAGCCTCAGTACAATGTGGAAATGGATACTGACACATAG
TGCCCCAGGCTTCTCCCACTTGCCCCGCTCCTTGCTGCTCTTCTTTCTTGTCTACTCGCTTTCTGTCACCTGCAATGAGGCCAGGGGCAGGGACTGGCTG
CGCTCCGCGGAGGTGGCTTCCAACTCCTCGTCCAGTAGGCATGTGCGGAGCTATAACCACCTCCAGGGAGACGTGCGGAGTAGGAAGCTCTTCTCCTACA
CTAAGTACTTTCTCCAGATCGATGGTAACGGCACGGTCAGCGGCACCAAGAAGGAGAATTGCCCTTACAGTATACTGGAGATAACATCTGTGGACGTGGG
GGTTGTGGCAGTGAAAGCCATTAACAGCAATTATTACTTAGCCATGAATAGGAGAGGAAAGATCTACGGTTCGAAAGAATTCAATATTGACTGCAAGCTG
AAAGAGAGGATAGAGGAGAATGGCTACAACACCTATGCGTCACATAACTGGAAGAATAACGAGAGACAAATGTTTGTGGCCCTAAATGGCAAAGGGATTC
CAAAGAGAGGACCAAAAACAAGGCGGAAAAATACTTCGGCTCACTTTCTTCCAATTGTCGTATAACCTCAGATGAAGGAAATGACATATTTCTGTATTAA
ATACTTCAGTACTGTTCTTCAAGAACCCGTAGTGTGTACTCGCCTCCTGCTTTGATTCTAACGGGGGAGGTCCATGATGGATGATGTTCCTACTTGGATT
TTAGAAGGACATTTTTTTATTGACTTTGTGGTGACTATGGCCAGATGGAGAAGGAAAAGCAGCCAGTACCTGTGTTACATAGAATTATATTTGTACAGTC
CGATTTAATGGATACAAGACAATCAAATCATTGATCTACTGGGAAAGTTATTTATGGATTACTTCTTCAAATCAAAGACTTTCATGGCTCATTCAGTTTC
AGCGCTCCCAATGAGCAGTGAGACGTGTAAGAATTTACTGGAAGCACTTGGGTCAAAAGTATCGACGATGTATTTTTTTTTTTTTTTTTGAAGAGTCAAC
GTGAAGAATGGTTAGGATTAAAGAAATATAAACGTACTGAAACTGTTCTAACAAAAATGAATATTATGGTATGCCTGTGTACTTTTACCTTCATCTCTTT
CTGTTTCTTTGTAAGTTATTTATTTAATAGAATGTTTGATACCTGTCTTCTGTGTTTAAATCGTTTGTCTAAAATTCCAAAAAAAAAAAAAAAAAA

5' EST: AL794255 [+]

acacagtcaagctgcgcagctggcagaagacgcagaagatttagaagcagcaggatctggagcacttggagatacccaggactttgctatggacataaga
ggtccctgaagctggtttgccaatcgagttatggatgttggtgccttgttctctgcacataggaaagtccagggtcccgttgggcagcagctccccagtt
gccatctacacgctctcatccttccagtatgtttctttccatgaggcatttgctgatgccgacagcctcagtacaatgtggaaatggatactgacacata
gtgccccaggcttctcccacttgccccgctccttgctgctcttctttcttgtctactcgctttctgtcacctgcaatgaggccaggggcagggactggct
gcgctccgcggaggtggcttccaactcctcgtccagtaggcatgtgcggagctataaccacctnccaggagacgtgcggagtaggaagctcttctcctac
actaagtactttctccagatcgatggtaacggcacggtcagcggca

3' EST: BX696936 [+]

acgatttaaacacagaagacaggtatcaaacattctattaaataaataacttacaaagaaacagaaagagatgaaggtaaaagtacacaggcataccata
atattcatttttgttagaacagtttcagtacgtttatatttctttaatcctaaccattcttcacgttgactcttcaaaaaaaaaaaaaaaaatacatcgt
cgatacttttgacccaagtgcttccagtaaattcttacacgtctcactgctcattgggagcgctgaaactgaatgagccatgaaagtctttgatttgaag
aagtaatccataaataactttcccagtagatcaatgatttgattgtcttgtatccattaaatcggactgtacaaatataattctatgtaacacaggtact
ggctgcttttccttctccatctggccatagtcaccacaaagtcaataaaaaaatgtccttctaaaatccaagtaggaacatcatccatcatggacctccc
ccgttagaatcaaagcaggaggcgagtacacactacgggttcttgaagaacagtactgaagtatttaatacagaaatatgtcatttccttcatctgaggt
tatacgacaattggaagaaagtgagccgaagtatttttccgccttgtttttggtcctctctttggaatccctttgccatttagggccacaaacatttgtc
tctcgttattcttccagttatgtgacgcataggtgttgtagccattctcctctatcctctctttcagcttgcagtcaatattgaattctttcgaa

---

Library:

Name: XGC-neurula

External Dbs: Unigene tropicalis cDNA library

Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from neurula. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Anatomy: whole organism	Stage: NF stage 13 to NF stage 21
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS107 Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: NotI

---

Usage:

RNA injection:
Probes:
Type Size Promoter Linearization Site Description sense SP6 AscI

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 BamHI

---

Suppliers:

Search for clone at: EXRC