Summary Attributions

Clone Name: fgf7-HAR399	Gene: fgf7	Species: Xenopus tropicalis
Description: EXRC Clone Number HAR-399. Xenopus tropicalis fibroblast growth factor 7. To generate probe for in situ and mRNA for injection. Clone previously called: TNeu035m16

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Sequence:

Full length sequence: CR855443 [+]

CTTGTTCGTGCATTCAGCTGCTGAGCGGTTAATTGCTTCCAAGTGAGGTCAGCAAAAGGTATTTATCGAAAACCATTGAACAACAACAGGCTCAACACAC
ACAGAAAACCCCTCTTGCGTGTTGATTTATGGAAACAATTAGGATTGTGCTGGAGTTTTTCTGAGTTGAGAAATAGTTTGTCGCTACAGAATCTCATGTT
GCAAAGAGCAGGCAGGAGAAATGGAATACGTGTATATTCAGCTGTTATCTGCAGGAACAACGAGTCCGTGCAGTCCTGAACACTACAACTGCCGATGTCA
CAATATCCTGATACTCTGAAGGGAACTGTGTTGAGAAACAGTTGAGAAGTCCAGGTTTTATGGCTTACACAGAAGACAAGAATACAAGCCTGGAAGTGTC
TTGAGTCAGGAATTTGCATCAACAGCACAGACTTCATTCAGGTCCTGGACCTAGAAACACTAATCCATCTGAGATTTACGTTTCATTATGATGTCCATGA
ACACCCAGAGTAGTGCACTATAATGCGCAAATGGATGCTAACTTGGATCCTTCCAACTTTGCTCTACAGACCATATCTTCACATAATATTCATTCTGGGC
ACTGTGTCTCTAGCCTGCAATGACATGACTCCAGAGCAAATGGCTATAAACGTGAATTGCTCCAGCCCAGAAAGACATACCAGAAGTTATGATTATATGG
AGGGAGGGGATGTAAGAATAAGGAAACTTTTTTGTCGAACGCAATGGTATCTGTGGATTGATAAAAGAGGCAATGTGAAGGGAACACAGGACCCAAACAA
CAGCTTCAGTATCTTGGAAATTAGAACAGTGGCAGTCGGAATCGTGGCAATAAAATGCATTGAAAGTGAATATTTTTTAGCTATGAACAAATCTGGAAGA
CTTTACGGAAAGAAATCATGCAATGAAGACTGCAATTTTAGAGAATTAATCCAAGAGAACAAATATAACACATATGCATCAGCAAAGTGGACAAATAATG
GGAAAGAAATGTTTGTTGCCTTGAACAGCAAAGGTTCACCAATGAAAGGAAAGAAATCCAAGAAGGAACACAAAGGATCCCACTTCCTTCCGCTGTCAAC
ATCCTAACCATAACCCTGATACTGGTTCCAGCAGATTATTTTTATTTTATTAATATTAATAATAATGCTAATAATAAAAAGTGGAAAAGGACACAAATAT
AAGCTGGATCTACTACTGAAACAAACAAGCTGGACTTGCGCATTTCAAAAGACTGTGGTCAAAAAAAAAAAAAAAAAA

5' EST: AL657337 [+]

cttgttcgtgcattcagctgctgagcggttaattgcttccaagtgaggtcagcaaaaggtatttantcgaaaaccattgaacaacaacaggctcaacaca
cacagaaaacccctcttgcgtgttgatttatggaaacaattaggattgtgctggagtttttctgagttgagaaatagtttgtcgctacagaatctcatgt
tgcaaagagcaggcaggagaaatggaatacgtgtatattcagctgttatctgcaggaacaacgagtccgtgcagtcctgaacactacaactgccgatgtc
acaatatcctgatactctgaagggaactgtgttgagaaacagttgagaagtccaggttttatggcttacacagaagacaagaatacaagcctggaagtgt
cttgagtcaggaatttgcatcaacagcacagacttcattcaggtcctggacctagaaacactaatccatctgagatttacgtttcattatgatgtccatg
aacacccagagtagtgcactataatgcgcaaatggatgctaacttggatccttccaactttgctctacagaccatatcttcacataatattcattctggg
cactgtgtctctagcctgcaatgacatgactccagagcaaatggctataaacgtgaattgct

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Library:

Name: XGC-neurula

External Dbs: Unigene tropicalis cDNA library

Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from neurula. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Anatomy: whole organism	Stage: NF stage 13 to NF stage 21
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS107 Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: NotI

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Usage:

RNA injection:
Probes:
Type Size Promoter Linearization Site Description sense SP6 AscI

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 EcoRI

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Suppliers:

Search for clone at: EXRC