XB-CLONE-4349898
Clone Name: IMAGE:7028176
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Gene:
gps1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080478 [+]
ATGAGTGACCCGGAACTGAACGGCTGAGGCCGATGCTCCTAAGTGGGGAGAAGATGCCTCTACCTGTGCAAGTGTTCAATTTGCAGAGCGCAGTGGAACC
AATGCAGATAGATGCAGACCCCCAAGATGACCAGCAGAATGTGCCTGACGTCAGTTATGTTGTGGAAAACCCTACTCTGGATCTTGAGCAGTATGCATCC
AGCTACAGCGGCCTCATGCGCATCGAGAGGTTACAGTTTATTGCTGATCGCTGCCCTCAGTTAAGAGTTGAGGCCTTAAAAATGGCTCTATCCTTTGTGC
AGAGAACATTTAATGTGGATGTGTATGAAGATATCCATCGCAAACTTACAGAAGCCTCTAGAGAGGTGCAAAATGCCCCAGATGCAGTGCCCGAAGGTTC
AATGGAGCCACCTCCTCTGGACACCACCTGGGTGGAGGCAACGCGTAAAAAAGCACTTCTTAAACTGGAAAAACTGGACACCGATCTTAAGAATTATAAA
GGAAATTCGATCAAGGAAAGTATACGAAGGGGTCATGATGATTTAGGGGATCATTATTTGGACTGTGGTGACCTAAGCAATGCTTTAAAATGCTATTCGA
GGGCTCGTGATTACTGCACAAGCGCCAAGCATGTCATCAACATGTGCTTAAATGTTATTAAGGTCAGCGTGTATCTCCAGAACTGGTCTCATGTGCTCAG
TTATGTCAGTAAAGCAGAGTCAACACCTGAAATTGCAGAGCAAAGAGGCGAGCGAGACAGCCAGACTCAATCTGTACTGACTAAGCTGAAGTGTGCTGCA
GGCTTGGCAGAGTTAGCTGCTCGGAAGTATAAACAGGCTGCAAAGTGCTTCTTACTGGCTTCATTTGATCACTGTGATTTTCCCGAGCTCCTTTCCCCCA
GTAATGTGGCAGTGTATGGTGGTCTATGTGCTCTTGCCACCTTTGATCGCCAGGAACTTCAGAGAAATGTTATATCCAGCAGTTCATTCAAGTTATTTCT
GGAGCTGGAACCTCAAGTCCGTGATATCATCTTTAAGTTTTACGAGTCAAAATATGCATCTTGCCTGAAGATGCTGGATGAAATGAAGGATAACCTTTTG
CTTGATATGTATCTTGCCCCCCATGTCCGGACCCTTTACACTCAGATCCGAAACCGAGCTCTTATTCAGTATTTCAGTCCCTATGTATCTGCTGATATGT
TCAAAATGGCAACAGCATTTAATACAACTGTGTCTGCTCTGGAAGACGAACTCACTCAGTTAATTCTCGAGGGGTTAATTAATGCACGCATTGACTCACA
CAGTAAAATCTTGTATGCCCGAGACGTTGATCAGCGTAGCACAACATTTGAAAAATCCTTACAGATGGGCAGAGAATTCCAGAGACGAGCCAAAGCCATG
ATCCTGAGAGCTGCTGTGCTGCGTAACCAGATCCATGTAAAATCCCCACCAAGAGAAGGAAGCCAAGGAGAACTGACACCAGCAAACAGCCAATCCAGAC
TCAGCACTAACATGTGACCGCAGCAGTTACCTGCACTCCTGACTCTTTATATAGAACTCTGTGGGGCTATTACATATACAGACGTGTGTACCACCTGCTT
GGGTCACTATCCCATTGGCCATCTCAGTTGTGTACATATGAGCTTGTCAGGGGCTGTGAGGTTTGCTTCTACAAATTGTTGGATCTTTTCATGCTTTCTG
ACGGTGATATAATTCTAAGTCTGTGTTCAGTGTTGGCTGCACAGAACCAGACAGGGCCAAGAAAGGACAAGTAGAAGATTACAGGGTGAAAGTGGACTCT
TTCTATTCTACTCCCCGTACGATTAGCAGCTTGTGTTAAACACCTCCTTCCCAGTGTCTAGTCCAGCAAATTCTTATACTACATTCCCTTTCGCCCCTCT
TCCCCCCCTTCTTTCATGCTCACTGCTATACTTTGGAATAGAGTTAACCTTTCTCTTACTGGACTGACCTTACAGAATGCCCTTTAATTTTATTCATCAT
CAACCAGACTGGACATAACTGCATGTGTAGTTGAACAAACCCGGCCCAAAACCACCCAGAAGCATTGATATTTAAATGTCCCTGTTTTATTTAGTTTCTC
TTTGGAATTAAACATGTTATTTGCGTGAAAAAAAAAAAAAAAA
5' EST: CF786142 [+]taattaatcccccccccccccatgagtgacccggaactgaacggctgaggccgatgctcctaagtggggagaagatgcctctacctgtgcaagtgttcaa
tttgcagagcgcagtggaaccaatgcagatagatgcagacccccaagatgaccagcagaatgtgcctgacgtcagttatgttgtggaaaaccctactctg
gatcttgagcagtatgcatccagctacagcggcctcatgcgcatcgagaggttacagtttattgctgatcgctgccctcagttaagagttgaggccttaa
aaatggctctatcctttgtgcagagaacatttaatgtggatgtgtatgaagatatccatcgcaaacttacagaagcctctagagaggtgcaaaatgcccc
agatgcagtgcccgaaggttcaatggagccacctcctctggacaccacctgggtggaggcaacgcgtaaaaaagcacttcttaaactggaaaaactggac
accgatcttaagaattataaaggaaattcgatcaaggaaagtatacgaaggggtcatgatgatttaggggatcattatttggactgtggtgacctaagca
atgctttaaaatgctattcgagggctcgtgattactgcacaagcgccaagcatgtcatcaacatgtgctttaaatgtttattaaggtcaggcgtgtatct
ccangaactgggtctcatgtggctcaagttattgtcagtaaaagccagagtctaacaccctggaaaattgccagagccaaaagaaggccgaggcgaagaa
aaggctcagaattccaattctggtacttgaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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