XB-CLONE-4350619
Clone Name: IMAGE:7025923
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Gene:
thrap3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785412
[+]
taattaatccccccccccccccgggcaggtctggagagatgtcagcaccaataggaacaggttataaaaggtttatggaagaacagaaaaataaggctgc
tgaactagagaaagaaaataaagaaaaggaaaagctgtctagtttggagagaatgaaggatcgtaacagtcctacagattttgcaatgaacgagctagag
aaggcttatcggaaaagccagtccccaaaacgtttcaaaatgcgagagggattggagaaatttgaaactgggcagagctgcgtttcgccaaagaggaagt
ggaacaggagaagaaaggtcgctccagaaaggattcagacagtgactccaaaaaccaagacccatatgaccctgccaaatggggaagagttgtcttccta
catctggcanntaaaaaaaaacgcacggaaaatcggaaagaaatgggatgaatgatccctttatttggggaaaaaccccaaaaagggaagaataagggct
ttcaaagaaaaggggggaaatctgccctcttaaagagggttttttcccccaaaaaaattttttgtggggcggcggcttttttttttttataaaaaagaag
aggncgcggcagcccaaaaagagaaggggaaacttacccctccccggtgtttattaaaaacccctcgcttgaaaattttttggggggcacaagaaagaaa
aaaaaattttttttttccttataggaaagaaaaatagggggttatttttcacaaaaaataaaaacattattatatctatatttcttaattgaagagaaag
aagggctccctcactagttccccaaacaaattgtatccctttttttagaagaaagaaaatttttgtgacgcgccccaacaatgttggaagaaaaatgggg
cctcacaaaagaaagaataaaaacttcttcctacttttt
3' EST: CF785411
[+]ttcctcatctctgttgcgtttctggaagtcattattgttgttattgttgttcccgggaaaagcacctctgccccatggccttgttccccgtgctcggtat
tgtaagtttccccgggctcgtcctctctcactggttccttgaaagtctttgttagccaagcgtgacttgtcaaagcttccagaaccctcctctttttcct
cactctctgcagggtactcctcatttccaggtgctcgcaaagaatgagaggaatgagaggaggaagaagaagagcgagaacgctcacgcactttcctgtg
cttcttatgctttttagatgatttcctggacttttctgttttttcgggtgacctctccctggagcgacttgaatccctagaatctcctgagtctctggag
tgatcacgcttggagtctttgtccttgtgttttttacgtcgttcgatatcttgccgtagatctgagggctcatctctatatttgctgtctcccttgtgtc
ctgattcctttagagtgcttagcttcttcatgcaaaaatccatgttttctaaaagcactgggaaaaaatatcgattcttcctgtgaatttcaggactctt
ttttgctcttcaaaaattccagatccttttgcccttttaggaattttaataaacgttcactccttgtcatgggaaaaggaccaaatgggggctctttact
ggatgcaccattgtaaaaaatatgctggaacaaaaaatttcagaagggcttcccctttaaggctttgcaaaacggaaaatggctgaaaaaataaaacttg
acaccccggagaccctttttgattgcttgggaaaacaaattcaagggga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: