XB-CLONE-4350878
Clone Name: IMAGE:7026024
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Gene:
irx5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785246 [+]
taattaatcccccccccccccgagaaaaagagagcagcagcaggaggcagcaagaactgggactgcttttctgtactgcaactggtggggttcatctcta
tgcatttgtgtccggaaaccctgacaagagctgggtcccagttttccttgtactgtgtgcgtctcaaccgataagagtttatatgcatgtgtcttgccat
gtcctatctctaaggctacttataccaaccatctgcttctttggctctgtgctcctgccctgcttacagcaacactgtcatctcttgacccaccactgat
tagtttgggaggtccccttttggccctgcctgtttaccttatcctcgatctccttctttctttgcccatcctccctggcttcaacgttttcccccaacaa
tgttagcgacccattctcttgccttttctacttaacttcggctcttttgccattccattctgccccgtgttttaccccaaatttacacacgatcctctaa
cccactaattttttgccctcccttccgggggcaatctcaacttacattaaccgcgcaatacctcattattttatctctttacacatcccggtttctaccc
tattatttctgcccgccatcctttatctacccgcctgttgantccggattctcattcttntattctaatatatccgccccttttccaccgtgccttggta
tacgttttcccgaatataccctgtcctccgttttattatattccccccccccatatttttatttcttacgccgccaggtcccactttccctttattcccg
gccttttctttatcctgctcatcctcccttgtcgcctttggtttgccctctacgcgtgcgccttgttttcctgtattgtcgtccatatgggtatcatatt
cgccttttgctgtttaaaaataaactattgtgtgtcccgcgtattaacaacacactgacctagtctaaaggttataccgtgtgagttttcgttaccgccc
tctatcccatatttatgttccgcgtcattttattcctattattttatctctcacaccctaccgattctaagccttgcttcttctgtatg
3' EST: CF785247 [+]tttttttttttttttgtttttgttttcataatttactgatattgatgatccatttaaatacaaattcgctacgtaaatatacagaatccgattaatacaa
atcaggtattttcttgcagttggattaagacatctgttaaatcaaagtaccaaaaatactttggatttcaaccgttaataattattttcttgatggtgaa
ggaatacactaacaaggcattgtatgttcaataaattaaaaaatggaagtccaaggcctgtagaggaagccaaaattaaatgctagacatacctttcttc
atttcataaggcgtgtctttgtttaggtcagcttgtgactggcttctaagtttgcactctttggccaaagcctcggctctgtttaatacagtctggttta
ttccactgaaatgatgggtgctgttaaccgttgggctagttgtgctaggtccatggtggctgtgaaggtgactgaaagtgccatagttcgtataaccagg
gtaaaagggattgctgtaataaaggggacgagacagaacggtgttgctggggaaatggcattgtgccgaaagtgatctggatggagaagaggcactaaca
accatagactgggctggagtggtccctgcaactgcagcagcatcgctgctttctttgactttgtccgaagaagttgctatctctgccagtgaccagagtt
taggctttgaggtggaagctggtgggggagtggataactgaangtggcattgtttggtgactgggccatgttgggacaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: