XB-CLONE-4350881
Clone Name: IMAGE:7026025
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Gene:
srsf12
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080452 [+]
GGCACCCCTTAAGGCTATCGTCTTGAGAAAATACTGTTCGGAGCCTGATTATAACGCTATTTTTTTTTTTAAATCGAAAAGCTCGGCTTTCTGTTATTGT
AGGGCTTTGGCGTTTGTCTGACTGGGGTTTAGAGTCGCAGGATGTCTCGCTACTCAAGGCCGCCCAACACATCCCTCTTCGTCCGAAACGTGGCTGATGC
CACCAGGCCGGAGGATTTGCGTCGTGAATTTGGTCGGTACGGCCCAATAGTAGACGTTTACGTTCCACTTGACTTCTACACTCGCCGCCCACGAGGATTT
GCTTATATACAGTTTGAGGATGTTCGTGATGCTGAAGATGCCCTGTATAATCTTAACAGGAAATGGGTTTGTGGTCGACAAATTGAGATACAATTTGCTC
AAGGTGATCGGAAAACTCCTGGACAAATGAAATCCAAAGAGCGCCATGCCTGTTCACCAGTTGATCGCAGGAGATCCCGAAGCCACAGCAGAAGGAGAAC
ACGTAGTCGGAGCTCCTCTTGGGACCGTGGTAGGAGGAGGTCTGGCAGCTTAAAATCATCAAGGCACAGAAGGCATTCTTACAGTCATTCAAAGTCACGT
TCAAAATCTCCTCAAAGGCGTTCCAGGACACCGCAGAGACATTCCTTCTCTAGAGGACGCTCAAGATCAATGGAGAAATCACCTTCTGGCTCACCTCCAA
AGGAATCTAGCTCTGGGTCAAAGTCCAGGTCAGGCCAAAGGCGATCTGGCTCCACTAGATCCCACTCGAAATCTCCTCGTCATAGACATTCAAACTCTTT
ATCACAAAAAGAAGCAGCCAGACATTCTAGTTCTCGTTCCCGGTCTCGGTCACGTTGTTATCGGGGCAAAAACAGTAGACAGTCTGGGTCTGACGAAGAC
TAGTGCATATTCTTGTACCTCTTAAGGTTGTTGCAAGTTTTGTCCACAATTGGCAGACTTCTCATCTGCCAAGAAGTTTACAATATCAGCTAGACTTCCT
TGCTTACTTTCTAAAAAAAAAAGTTAAAACATTACATGTGAGAATGGGGGCAAGCAGCTCTCATAAAGTTTCTTGGGCCCTCTAAGACCTCCGTGCATAT
TGTGTGTGTGTTTTTTTTTTTGTTTGAAACACCTGTAAAGCATTCTTTTTAGAAAACATATTAATTTTGTCATTTGGTTGGACTGCTGAATGTTTCAAAG
TATTCTCTGGAGTGTGTTCCTGAAATATATGCCAGTGCTTTGTTTTACTGTCTAGACCAACCACCTAATATTTATCAAAAAGTGTTAAAATGAAACATTT
TCATAAATGTAATTGTGTAAGGGAAGATCTGGCAAGCTTGCTAGGCTTAAACCGAATGAGGGTACAATTTTGTCGTTTAGTGCAAGGATAGTGAGAATCT
TTCCATCTTATTAAAATATTTTGATACATACACATATATGTGTAAAACTAACACTGAAATCTAAAGATTGATATTCATTTCAGCAATAAGGGTTGCGGCA
CCAAGTTTTACAAATGTGCTTTTGTTGAAGACGTATGTTTTACGATTGTAAATGACAATTTTTATTTTCTGGAAAAAGCTGCACAAATACAGGTTTTGTT
TGTTTACTGTGCTGCATAACCATTGTTTTATTGCTTTCTGCAGTGTTTTCTGCATTAGTCAAGTATTTTTTAGATGCAGTAAATAGTTTCATAATGCTTT
GATTCTGCCTCAAGTGCTGTCACTTGAAATGTTGGACAGTTGAGTATTTTGTCCAATGGTTTTAATAAACTGAAAGTTTCCAAACGAAAAAAAAAAAAAA
AA
5' EST: CF785245 [+]taattaatcccccccccccccggcaccccttaaggctatcgtcttgagaaaatactgttcggagcctgattataacgctatttttttttttaaatcgaaa
agctcggctttctgttattgtagggctttggcgtttgtctgactggggtttagagtcgcaggatgtctcgctactcaaggccgcccaacacatccctctt
cgtccgaaacgtggctgatgccaccaggccggaggatttgcgtcgtgaatttggtcggtacggcccaatagtagacgtttacgttccacttgacttctac
actcgccgcccacgaggatttgcttatatacagtttgaggatgttcgtgatgctgaagatgccctgtataatcttaacaggaaatgggtttgtggtcgac
aaattgagatacaatttgctcaaggtgatcggaaaactcctggacaaatgaaatccaaagagcgccatgcctgttcaccagttgatcgcaggagatcccg
aagccacagcagaaggagaacacgtagtcggagctcctcttgggaccgtggtaggaggaggtctggcagcttaaaatcatcaaggcacagaaggcattct
tacagtcattcaaagtcacgttcaaaatctcctcaaaggcgttccaggacaccgcagagacattccttctctagaggacgctcaagatcaatggagaaat
caccttctgggctcacctccaaaggaaatctagctctggggtcaaagtccagggtcaaggccaaaaggcggatctgggcttcaactaggatccccacctc
gaaaatcctccctcgtcaataaaaaaatt
3' EST: CF785244 [+]tttttttttttttttcgtttggaaactttcagtttattaaaaccattggacaaaatactcaactgtccaacatttcaagtgacagcacttgaggcagaat
caaagcattatgaaactatttactgcatctaaaaaatacttgactaatgcagaaaacactgcagaaagcaataaaacaatggttatgcagcacagtaaac
aaacaaaacctgtatttgtgcagctttttccagaaaataaaaattgtcatttacaatcgtaaaacatacgtcttcaacaaaagcacatttgtaaaacttg
gtgccgcaacccttattgctgaaatgaatatcaatctttagatttcagtgttagttttacacatatatgtgtatgtatcaaaatattttaataagatgga
aagattctcactatccttgcactaaacgacaaaattgtaccctcattcggtttaagcctagcaagcttgccagatcttcccttacacaattacatttatg
aaaatgtttcattttaacactttttgataaatattaggtggttggtctagacagtaaaacaaagcactggcatatatttcaggaacacactccagagaat
actttgaaacattcagcagtccaaccaaatgacaaaattaatatgttttctaaaaagaatgctttacaggtgtttcaaaccaaaaaaaaaacacaccccc
aatatgcacgggaggtctttagagggcccaagaaactttatgagagctgccttgcccccaattctcacatgtaaatggttttaacttttttttttttaaa
aagtaagccaagggaagtctagcctgatattgtaaaacttctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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