XB-CLONE-4350922
Clone Name: IMAGE:7026810
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Gene:
krt12.5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785220
[+]
taattaatcccccccccccccggcatccttgctctctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctata
ggtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttggagg
aggctttggcagcggtgcaggctctggcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtggaaat
gaaaagcacgccatgcagaacctcaatgaccgccttgcttcctacctggagaaggtcagagccctggaagcctccaatgccgacctggaagggaagatcc
gcacttggcacgagaagcaaacaggcaccggccttggagctgggtctaaagactacagcaaatattttgagatcatcagtgagctgaggggcaagatcca
cggtgcaactgttgataacgctacagtgaccctgcaaatcgacaacgccagactggctgctgacgacttcagacttaagtttgagaatgagctggctctt
cgccagagcgtggaggctgatatcgctggcctgcgtaaagtcctggatgagctgaccctggctagaggtgacctggagatgcaaattgaaagcttgactg
aagagctggccctacctgaaaaaaaaaccctgagggaggagatgatgccatgcccaagaacccaagaccgccccgaaaaaattggattggtttgagaatg
ggacgcccggccttccagggc
3' EST: CF785219
[+]tttttttttttttagagttaaagatcttttattgctttatgagacatttccataatcatttcttctttcttacgcaggtcttctctcttgtttcagggga
aatttctgttcatttgttcctttatctttcccgactgcccttggatttatgacactgttgaagaaacaaccttcccatctaccacctcttcgacaatggt
ttttactgttctggttctggttgttgttgttgatgatgattctgagctggactccagggaacttgcctttgacactgtggtcatctgtcccagttcccca
tccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgatgttccatgtcggacctga
tctgcagaagctgttcctctaagctgctgataatgttccgaatctgtgaaaatgcggctgaaaaaaagccttgaaaattcattggagatgcctttaccct
gaattgtttcatttgccacctgggcattgcacaatcaatttccaagcatctaaagtggaccgtttgcggcaaagggattttcgtttcttggcggggcatc
tgaatttagtttaaaccacgaatagaaatttccttttttttatgctcagaggctcatttttggggttagagccgtacctatggttttctctaagggtgtc
tttaccagaccagctattttatgagatacgtgtggtgacgatatattccaacgagtttctgcccaatgtacaacaatacctaaagagatcagtccacaat
tctatatatacttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: