XB-CLONE-4350930
Clone Name: IMAGE:7026813
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Gene:
cirbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785215 [+]
taattaatccccccccccccccgacttcgcattcggcttccaacttgtgttgtatcgtccgaagatcatagaacgtctcaccatgtcttgtgatgaagga
aaactctttgtcggtggtctgaattttgaaaccacagaggaaagcttggagcaagtgtttagcaaatatggacaggttgctgaagtggttgtggtgaagg
atcgggaaagcaagagatccagaggctttggctttgtcacatttgagaatcctgaagatgccaaggatgctatgatggcaatgaatgggaagtctgtaga
tggccgtcaaatccgtgttgatcaggctggcaagtcctccaatgataggagaggtggttacagaggtggctcttctggaggcagagggttcttccgtgga
ggcagaggccgaggtggtggtggagacagaggatatggtggaagcagccgctttgaaaacagaagtggaggttaccaaagcagtggatccagggattact
atggcaggagtcatggaagctatggtgatcgttccggaagatcctacagggatagttatgacagctatactacacaagagtaaaatccattcctgactca
agatcgtccttccaatggctgtatttataaagattttttggagcttcgcccaatcgttttgtgtagtatatctacttgtgttcactttttttttttaata
gaccctatttagcctgaacagctttctcctaataaagtggggacaacctgtgtttttcaaaggcttttatccttttgggacaacctaaaactccctttct
taaaaggttacctgggggtt
3' EST: CF785214 [+]tttttttttttttttcacattggtttttttaataggcttcgtgaagccaatcaaaagtagaatacaattccttagccacttcagtacaatagttgcatgt
ttagtaaaaaaaggacaatataaacaaacattttaagaacttcttctgatattgttactggagatgcgaacactattcactttgtgtatcaaggaggtgt
agccactatatacagcgcacccaagctctgccttgccttgttacactaaaggaatgcaatttaagtggactcataaaattaacttttcatgtaaccaaaa
aatacagcagcttaaagttctttaaacaatgctacaggaagacaggcctagcagtctgctactaatacaggtgcttctctgcccatcaaccaacagcacc
catccgcttaggaagttacttcctagcactacttaaccagaagggggagcagccccacctgatcagcttgaatggaacagcatagtaggataggaatatc
cttgcagaacacagtaactttaagaatgagattaggtgtccaaagataaagcctttgaaacacagctgtcccacttattagagaagctgtcaggctaact
aggtctattaaaaaaaaaagtgaacacaagtagatatactacacaaacgattcggcgaagctccaaaaatctttataaatacagccattgggaggacgat
cttgagtcaggaatggattttactcttgtgtagtatagctgtcataactatccctgtaggatcctccggaacgatcaccatagcttccatgactcctgcc
atagtaaatccctggatccactgctttgga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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