XB-CLONE-4350947
Clone Name: IMAGE:7026821
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785204 [+]
taattaatccccccccccccccgcaatcagagagctgtgctttttatgaacggtgttttgtgtgaggtgtgtgcggacagctgagcgtgggcccataata
atgtagaaccgagccgcgggcgagagtctgcctaatcttgcatgtggatggtccggctgtgtctgtcccgcaacagcttcaagcggggaaagtgcaaagc
taatctggtccttgctgcagactctacacaggagcatagtaggagaagatgcaggctgttagggaagctcatgtgggaggtgaaaacaagtcctttgtaa
aacagaagagcagctgggaaaaaggaaaagaaaattgcatggggccaccagcatgtcgtccacaaagcaaggaaaagcaaaccatgcaagaaaatatcct
gaaaattcctcagaaaagcaaacttccagtacttgcaaaaaccaagccaccccctgactttcagaaaatgcaccaagtgtggcagaaccagtttcagaag
ggtaaagccatctctaaaaagtcctgcacccgtcctcggcctttcaatttgtctcagaaaggagacaagtttcgcatagcatcagtcactgatgtcggac
accctgtggcaacttcctccaacatgaaatctcctgtcgcaggccttacaggtcgagagccattaagtgaagtttttcatggacaaaataacacagccaa
ttcaaaagatggttccacgatgcactttaaagctgaccctgcagctcttaacagtatcattttttaatgtttgggatatctactggctgggaggaacaac
ctgggaaaactgaagcttgggcacagagggttcccat
3' EST: CF785203 [+]tttttttttttttttagtgtataaatagtgttttatttttaaagaatataaaatttaaatacgcattaaaaggtattagaaattgggaattatcttgctc
aaaaattacattgtacatatacagagcatatgatacaaacagaaatggaaacaaattcatttggcaactttatcactactgttccaaatctgctccgggc
agctatttccaaacttcctttcttcagcctggctaaaagtgtctcaggccancgagcagcgtgtgcagaatgagcaaagggaggcaaaatctaaaaagaa
tcagggactctccaacttggaaatagggaataaaagtggcaagaattttcctgcctttttcccaaaaggtaaaaagcctacgggggtcaaataacaggcc
tccccggggggggttccccctggggggcccccccaaaaaattgtgtttaaaaaaaagccccattttcccccccaccccaagggcaaaattggttccccca
aaaaaaacccggctttggtttttaaaccccgcaaaacccccgtgttgtgccacaatgggtaaggggcccccaaaaaaatcccgggggcccatattgtgtg
gggagggctattaaaagggggtgggggggaaaaaaaccccccccaaaaaaattggggccccttttgaaaaattgggggcggcacaaaaaaacccccgttg
ggttaacaaaaaaaaatatcttttttaaatataacggggccgcccaaaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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