XB-CLONE-4350961
Clone Name: IMAGE:7026826
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Gene:
arfgap3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785195
[+]
taattaatccccccccccccccgggagtcggtgagtgagaccggagggagcggagagagaatggcggaaccgcacaaacaggatatcgcggccatattcc
ggcgcctccgctccattcccagcaacaaggtgtgctttgactgcggagccaagaaccccagctgggcgagtatcacctatggggtgttcctgtgcatcga
ctgctcggggacccaccgctcccttggggttcacctgagcttcataaggtcgactgaactggattccaactggtcctggttccagctgaggtgcatgcag
gtcgggggcaatgccaatgcgacagtcttcttccggcaacacggctgcgccaccaacgacaccaacgccaagtacaacagccgcgcctcccagctgtacc
gggagagggtgaaatcccaggccacacaagccacgcggcggcacggaactgacctgtggatcgacgcttgcggggccccaccgctgtcaccccaacacca
agaggaggactttttcgctgctcattcagagcaggataataacacatcgcaggcccccccccaggcagaggacacccccataaacgtgagctctgaaagt
ggccccgccaacaacgaaggggagccggaagtgggtcccagcgtcgactacctcagcgtctcgcccaaagccgcattagaatccgtctctgttctaaaga
aaaacccggagccgccaagaaagggctcgggtgccaagaaagggggtctcggggcgcaaaaagtgaacg
3' EST: CF785194
[+]tttttttttttttttcttttttttttttttttttttttaagttgcaatctaacctttattacagagtacaaatcgatacaaagatggcgtcggtattgtc
agaggaaccgcccacaggaaatcaccacacacattaaaaaaaaaaagagaattttggccccgccccctccaagaacaaaatgaagcctgacaatacccat
aatgctgtctaaaacacagcaagtttcccttccgttaccatggaaaccaggatccttattcctaaactaaacgagggctactaacgagcaaatagatttc
cttttatttcaagtaaaattaatattgtaacttgcaaattgtatataatatttccaatataggtgggaggggtctgttacctggaaacccgccccccaga
acccccagctgctgagcattccggataacagatcccacacctgtgtgtatgcgtgtatatacatatagattcattcctataaatattggggtgcagcgac
tgcaaacgcgccccccctcttctctaagcaaagatacaaacgcgccaactggacttccattcccagaagcctttgctggtttctgaggggggcactggtt
gtacagaatgaggctcggggggcctctgattgtccagcaacgcaattaaggctctaatgacgggggtggagcctggggtgatgggggagaaccccggggt
gggggcactgggggctccataaaaatggaacatgtgccccccccatgttaatgcccccatctgccccctttgtcccaaacgcagaagtaaaagcttaatt
caaggaaaacgaaccccaaaagcggaagcgggggatggccccaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: