XB-CLONE-4350964
Clone Name: IMAGE:7026827
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Gene:
atp5mc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785193
[+]
taattaatcccccccccccccgctttccccgccaagcagcgagtgctattcttgttcctgcgtcttgttcactttcaccccaaatatgtacgcctgcgcc
aagcttgtgtctaacccagccttggtgagaagtggagtctgtcttctctctcgaccagtttctgtcccattactgagctacactggactgaggactgagc
agctgatgcctgttccaactaggggaatccaaatcagtgcaacatgcagggacattgatacagctgcaaagtttacccggaccggtgtcgctacattacg
tgtgggctggctttagcgatgggaacgggacattgcttgcgcccctgattcatcgcgtcggctaggattccttaccattaaacggcaatcgcggggtacc
gtgtcccgtttcgggcttaactcttcgcgccacgatatacgcgcatcgtttgttgctcccccggcgtattttacctttacaaccaacgcggttatgattc
ctttatccttttacattcttcccttatccatcccttttaccggatcgtaaatactctataaaagaacttatcgttacggagctattattccctatcttta
tacccccccccttactatctttttattttttttcctcattttacacctctattgttgcttacacattgaaaacacgattctccgtgtgctcgattttatt
atcaccattttcctcntctttttaacacaccacccaccctctttgcactatacaacaaggtgttttcgtcgcatttctcccttctcttttcgccctgacc
gaaattgcgggagcgcgtaagcagaacccacattatattagctccgcccctccttctctatgagttccctgctcccccgcttcattttttttaacttata
ttcgcggaattccatattactcactcgtgtattagtgtggcgcgtccactcttcggcgtgttgtccctctccgttctgcgtgtcacccgaccccatgtgt
ttttttatcgcgcccgcgccgcccgcgtattattcgtcgacgcgtgtattcacatatacgcggcttccccgtattagaaataatccattccaacgcgtct
gttccttgttattttatataccccctaccctccatatttttctcccccgcgaggcgcgctatataaagcgcg
3' EST: CF785192
[+]tttttttttttttttatttttttttccaggtgaccactttaataataccatctgtccattcaaagaattttatgcacacatctacttttttagagtgagc
tcctgaaatatgagacccacaatgtatttataatatttattccccaggaacatcagattatacagcagcagagtgaagagggatctaccctcaccacaaa
gccctagtacagggggtccaaaatataatctggtgatcttaatgttttacatggcaaacaggatgaggaaagcgaccataagacagaacagtcccatagc
ttcagacagggcaaaccccaggatggcataagagaagagttgttgtttcagagaaggattccttgcgtatccaatgataaggctgccaaacactgtccca
ataccagcaccagagccagccacacctacggtagcagcaccagctccaataaactttgcagctgtatcaatgtccctgcatgttacactgctttggattc
ccctagctggaacaggcatcagctgctcagtcctcagtccagtgtagctcagtagtgggacagaaactggtcgagagagaagacagactccacttctcac
cagggctgggttagacacaagcttggcgcaggcgtacatatttggggtgaaagtgaacaagacccaggaacaagaatagcactcgctgcttggcggggga
aacgggggggggggggggggatttaaataaacccgaggggga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: