XB-CLONE-4350967
Clone Name: IMAGE:7026828
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Gene:
tnnt3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785191 [+]
taattaatcccccccccccccccccggcgattgaagagtctcacaactgctgcgacccccagtgctgctctctctcgggcctcaggtgaccaaaagccat
catgtctgacacagaggaagttgaacaggtggaggaagaatacgaagaagaggcacaagaagaagaaagccaggagcaagaagaagaaccagttgtggaa
gaggcaccacaagaggaggaagcttatggggaggctgaggaggaagaacatggtgaagaatatgatgaggagaagccaaaacccaaactgacagccccta
aaatcccagagggagaaaaagtagactttgatgatatccaaaagaaaagacaaaacaaagacttgattgaactacagtctctgattgacacacattttga
ggcccgcaagaaagaagaagaggagctcattgccctgaaagaaagaattgagaaacgcagagccgagagatctgatcagcagaggatcagagcagagaaa
gacaaggagcgtcagaatagattggctgaagagaaggcaagaagagaggaacaggatgccatcaggcgtgcagaggatgacatgaagaagaagaaggctc
tgtcctccatgggtgccacatacagctnnctcctggctaaagctgatcagaagagaggccagaaaccaaccgcccgtgagcaaaaaaagaagatttgggc
tggaccgtccaaaccactgaatgtccaccccctgaaatgatggacaagctcagggaaaaggcaaaggaaatgtggggactggc
3' EST: CF785190 [+]tttttttttttttttggggtaagaagcatcacactttatttacaaaagggaagagataataaagcagtccacagagagaacttggtggcacaaaagggaa
atggcccttgagggtgcatggagtggaaccagcttcctaaacgagtactgaagaaatggcagaaaggggctgctctctgtgaggaccgggtgttgatttc
gagttgatttacttccagcggcccccaacttttcctttgcctccagctgccttcttgctgaacttggagagttcctctactctcctgtgcagagtcgtaa
tctcgtatttctgtttcttcagcttctctcccatctcaaacttttcagactccaactggtacagccagtcccacatttcctttgccttttccctgagctt
gtcatcattcatgtggtcgacattcagtggtttgcgacggtcagccagaatcttcttcttttgctcacgggctgtctgcttcttgcctctcttctgatca
gccttagccaggtagctgctgtatgtggcacccatggaggacagagccttcttcttcttcatgtcatcctctgcacgcctgatggcatcctgttcctctc
ttcttgccttctcttcagccaatctattctgacgctccttgtctttctctgctctgatcctctgctgatcagatctctcggctctgcgtttctcaattct
ttctttcagggcaatgagctcctcttcctccttcttgcgggcctcaaaatgtgtgtcaatcagagactgtaatttcaacaaagctttgtttttgcctttt
tcttttgggaaatcatcaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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