XB-CLONE-4350984
Clone Name: IMAGE:7026834
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Gene:
sumf1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785180 [+]
taattaatccccccccccccccgggcaaactaaacatggctgcgcccggccggtcagactactccactctccagttactagcattgttgcttccgctgct
gggaccggcaaaggttttagctggatgcgggtgtggcgataccagccgagacggtcacggtcatggacacggacacggacacggacacggacacggcgag
gggttggacagtccgcacagaggcgctgccttcaaatactcacgggaggccaatgagccggagcccgcacagaaacagacccagggacgggagcagatgg
ttctgattccctcaggngtgtttactatgggtactgatgagcccggtatacctcaggatgggggaaagtcctgctcgcaaggtgcgccctggattctttc
tacatggatacctatgaaagttagcaacctggagtttgaaaagttttggaaaaaccacagggccatgttaacagaggccgaaaaattttgggggatcctt
ttggaatttggaaggctttgataaatgaaaaatgttaaaaaagcccccttccattccgggctttttcctgcctgccccccctggttgggccttcccggtg
aaaagggaaccttaaatgtggaaaaacacccctgaaagggggcaaaaaatctttaacattttttaaaaagggggagggggatcatccccgggggtccctt
caaggg
3' EST: CF785179 [+]tttttttttttttttcaatcacaaaaataattttattaataaaataggattttgtgttcattcttattatgaaggacaaggaatgtcattgaaatttttg
ttttcacaaggtcttgggagttccttcctgctcaggtcattttgcagtggtcacggagccgacgccacgcagcggaatcccaggttagaggccgagctgt
caggtgtattctgacttcgagcagcacagcgatacctgtagcagtaggactcatggcacatgtatgagcctccttttttcaccttgtcttttcccgtaga
aggacctttcgggttgtaagtctcatctgctgtatgatgagtgtcccaccaatcggatgtccactcccaagcatttcccaccatgttatataggctataa
ccattggctgggaaagcggttacaggtgaagtctgcacatagccgtcctctccagtgttttgggttggaaattccccctgccagacattcgcataatgtt
ctccctttggttccagcttgttcccccacggaaaaagcctgttctcaagtcccccacgggaggcatattcccactcagcttctgtcggaaggcgcttacc
cgcccaggtgcagaaggcagaagcatcgttccaggacacatgaagaacaggatgatccatcctgtttaagatgttagagtctggcccttcagggtgtctc
cagtcagctcctttcacaggaagccnacagggcgcagcagcaacagcctgatcgatggtgcttttcacatcttcactaatcaagcctttcaatacaaaaa
gatcaccaaaatttttcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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