XB-CLONE-4351014
Clone Name: IMAGE:7026847
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Gene:
myh4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785161 [+]
taattaatccccccccccaccccccctgtgattgaggaccaacttagtgaagtgaaggcaaaggatgatgaacatcagcgtctcctcaacgacctcagta
ctcaaaaggctcgcttgcagactgaaaccggtgagctctcccgccagcttgaagagagggaatctctgatttctcagctctcaagaggaaagcagggatt
tacccagcaagttgaagaactcaagaggcagcttgaagaagaaacaaaggctaagaatgcccttgctcatgctctgcaatcagctcgtcatgactgtgac
ttgcttagagaacaatttgaggaagaacaggaggccaaggctgaacttcagcgctcactgtccaaagccaatggtgaagtttcacaatggaggaccaaat
atgaaactgatgcaattcagcgtactgaggaacttgaagaagccaagaagaaacttgctcagcgtttacaggacgctgaggagcaagttgaggctgtgaa
ttccaaatgcgcctccttggaaaagactaagcagagactgcaatccgaaatggaggacttgatggttgatgttgaaagagcaaatagtgcaacagctgct
cttgataaaaagcaaagaaaactttgataagggccctggttagaatgggaaaccgaaattatgaaagaggggccagggcctaaattgggaaaaccacccc
ctttaagggaaatcctcgcagcccggggagcacctgaaaattctttccaagaaaagaaaaaaatggccctttattggaagggaaaccttccgtggggaac
acattattttt
3' EST: CF785160 [+]tttttttttttttttaagcttcaagtaatttatttacaattcatttgatacattatatttggaatggacattacaatttcacattccttagccttctctg
ttttcactcttcagactctttcttgcctccaatatcacggctctttgtcctgagcttattcacttgggactcggcaatatcggctctttcttctgcttct
tcaagctcgtgctgtaccttacggaagcggcccaaatgggtgttggcttgttcctcagcttcttcagcctgcctcttgtaggctttaaccttagactgaa
gtttatctaccaggtcctgcagtctcaaaatgttcttcttatcttcctcagtctggtatgtgagttccttcactctcctctcatatttacgaacaccttt
cacagcctcaacaccatgtttctgttcattatccagctcattctccagttctcgtaccctggattccagtttctggagctgcttcttgccacccttcatt
gccagctgttcagcttcatcaaggcgatgctgcaggtccttcacagtctgttcaaggttcttcttcatcctttctaagtgagcactggtgtcctgctcct
tcttcaactcttctgccatcagggcagcatctgttatggccttctttgccttctcttcagcatttctggcttcttggacagcttcctcggcctcattctg
tagttgacagatgtcacttttcagcttcttcttgctgttaatgagacttgtgttctgggaaatgcacagctgaagacgttcacttgcatctaggagctcc
tgttctgccatcttacan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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