XB-CLONE-4351020
Clone Name: IMAGE:7026849
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Gene:
commd4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084516
[+]
GGAGAAGATTATGTCACATGGTGCAGCAGGGCACTAATCAGCTGACAGGAAGGGTTATACCTGCTTTCAAAACATGAGGTTCCGGTTCTGTGGTGACTTA
GATTGCCCTGACTGGGTTTTGGCTGAAATCAGTACCTTATCCAGAATATCATCAGTTAAGCTGAAGCTGATCTGTGCCCAGGTACTAAAGAAACTACTTG
GAGAACAAATTGATTATGAGAAAATCATCAAACTGACATCAGATGCTCGATTTGAATCAGGGGACATCAAGGCTACAGTTGCTGTTCTCTGCTTCATTCT
TTCAAGTGCAGCTAAGCATAATGTGAGCAGTGACAGTCTGTCAAGTGAGCTGCAGCAGCTGGGACTGCCCAGAGAACATGCTGCCTCTCTGTGCCGATCA
TATGAGGAGAAGCAAAATGCACTGCAGGAGACACTTAGGGAAAGCAGCCTGCGCTTGACACGTATATCCTCATTGAACTGGCGCGTAGATCAGATCCTTA
GTTCCAGTATTGTGAAGCAAGTGAATGAACCCCTTGTGCACCTTAATCTGAATGTGACAGATGCAGGATGCAACCAACCTCTTACCATGACTGTTTCTGC
TTCCAAACTCCGTGTCCTTATAACAGAACTGAAGCAGGCTCTTGAGATGATGAATGCCCTGAATTAAGACCAGATCAGTGGCTCAGCCAGCTTTATGGGT
CACAGTCAACAGATGGAGAAAAACTGCAGCAAGAATGGCATCAACAGGTGTACTTTTACTGGGCAGAGCAGAATATATAGAGCAGCATTTCAAAACAAGC
TCAGTACAAAGTGAACTGTAGCAGACCCTGCAGTTATGCCTTTCTTTTCAAAGGGACATTATACCTGCCAGTTATGGCAGTTTTGAAAACCATATACATT
TCTCCTGTCTTTTTCATATGTAAAATGCTTTGGTAAAACAAAACAGCATTCTACCTTTTGTTAAAAACTTTCCCAACATAGGCCATGGTTTCAATGTTCT
TTGTATGAGGTTACCTGGACGGAGCCAGCTATTGCTGTTCTCCTTTATGTACATAGACATACATCATGCAAGTAAACAGCACCCTGCCAACCCAATACTC
CATAACTTTTCCCAATATATTTCACTACAAATATATAGACCACACTAGAAATTAGTGCTTCAGAGGCTTAATTGTGTCAGTGCACGTAGTTATGATGTCA
CCCAGGGTGACCGACATTGTAGGGCATACTATTACTATTTCTACTGAGTTCGTGTAAATACAGTCTACAAGAACTGCAGCTGCAGCTCTCGGAATTCTCG
GTCTTATCCCCTACTACTTTTGTGTTTTCATCCTGATGTTTCCTTTTTATAATGTTTTAAGCTTGTATATATGTATTTGAATATGATGATTTGCTGGTAT
TATTACAATCATAAACTGTCTATTAATTTCTGAAAAAAAAAAAAAAAA
5' EST: CF785157
[+]taattaatcccccccccccccggagaagattatgtcacatggtgcagcagggcactaatcagctgacaggaagggttatacctgctttcaaaacatgagg
ttccggttctgtggtgacttagattgccctgactgggttttggctgaaatcagtaccttatccagaatatcatcagttaagctgaagctgatctgtgccc
aggtactaaagaaactacttggagaacaaattgattatgagaaaatcatcaaactgacatcagatgctcgatttgaatcaggggacatcaaggctacagt
tgctgttctctgcttcattctttcaagtgcagctaagcataatgtgagcagtgacagtctgtcaagtgagctgcagcagctgggactgcccagagaacat
gctgcctctctgtgccgatcatatgaggagaagcaaaatgcactgcaggagacacttagggaaagcagcctgcgcttgacacgtatatcctcattgaact
ggcgcgtagatcagatccttagttccagtattgtgaagcaagtgaatgaaccccttgtgcaccttaatctgaatgtgacagatgcaggatgcaaccaacc
tcttaccatgactgtttctgcttccaaactccgtgtccttataacagaactgaagcaggctcttgagatgatgaatgcccctgattaaaaccagatcagt
ggctcagccagctttatgggtcacagtcaacagaatggagaaaaaactgcagccaggaatgccatccaacaagtggtactttttactggggcagaaacag
aaatatataggagccacc
3' EST: CF785156
[+]tttttttttttttttcagaaattaatagacagtttatgattgtaataataccagcaaatcatcatattcaaatacatatatacaagcttaaaacattata
aaaaggaaacatcaggatgaaaacacaaaagtagtaggggataagaccgagaattccgagagctgcagctgcagttcttgtagactgtatttacacgaac
tcagtagaaatagtaatagtatgccctacaatgtcggtcaccctgggtgacatcataactacgtgcactgacacaattaagcctctgaagcactaatttc
tagtgtggtctatatatttgtagtgaaatatattgggaaaagttatggagtattgggttggcagggtgctgtttacttgcatgatgtatgtctatgtaca
taaaggagaacagcaatagctggctccgtccaggtaacctcatacaaagaacattgaaaccatggcctatgttgggaaagtttttaacaaaaggtagaat
gctgttttgttttaccaaagcattttacatatgaaaaagacaggagaaatgtatatggttttcaaaactgccataactggcaggtataatgtccctttga
aaagaaaggcataactgcagggtctgctacagttcactttgtactgagcttgttttgaaatgctgctctatatattctgctctgcccagtaaaagtacac
ctgttgatgcncattctgcggttcccgtagactacgaccgtgttgccacttagctatggaaggtn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: