XB-CLONE-4351050
Clone Name: IMAGE:7026860
|
Gene:
hhip
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF785138 [+]
ggccttgaattcgaatcgatctatacaatgtaacttgtgagatccagcaagtaagacgtcatgtcaatgatctgatcaaggatacctgccctggtcactc
tgcggacgttcctgtccacttgttcacattgaggaccaagatagccctttttacagaggcacttgttcggtctgacacagacacctccatgacggcacgc
tggctcacacttggcaattctgcagaagtctccctcccagccgggactgcagcagcacttgccagtgggggtgtagtagccgtttcgacagagccgggag
caatcggaggtcagtggctgggcaggttgaactgtgactctgcattcctcaggcattaaaggtcttttggggtctacgatcttgtagagttttccattgt
gagtctgggtcatactcttactgcttgatagaatgtaaacctctcctaattcatcttctccaaatcccaagatgtgacccgaaaagtagcctcgacagga
gctgctggcacccaggcagagcggcttttcttgccattgcttggtcactgggctttgctggagggttaagaaattcccattgcgatctccgaacacatag
cttccgtacaatctttcagactgacagcctctgtaaacaaatccaccaaccaaagggccgttactgaattggcttgaattcaagaaaagatggctcactt
tcataatctcttcccctttaatatctgtaagaattctgggctgaatgacctgttttttcccgtttggaatctggagcaaaagtaattgttaaaaattgaa
ggttttataatcaggtaaggaatgtcgcatcct
3' EST: CF785137 [+]ggcagacgatgctgaagatgctctcgtttaagctgctactgctggccgtggctctgggcttctttgaaggagatgcgaccatggagcaaaagctgatttc
tgaggaggatctaaacccccatatgcacatgggaaagtttggggaaaggagcgaggggagcggagcgagaaggagacggtgcctgaatgggaacccccca
aagcgcctaaagagaagggacaggcgggtgatgtcccagctggagctgctcagtggaggagagatcctgtgtggtggcttctacccacgagtatcttgct
gcctgcagagtgacagccctggattggggcgtctggagaacaagatcttttctgccaccaacaactcagaatgcagcaggctgctggaggagatccaatg
tgctccctgctccccgcattcccagagcctcttctacacacctgaaagagatgtcctggatggggacctagcacttccgctcctctgcaaagactactgc
aaagaattcttttatacttgccgaggccatattccaggtcttcttcaaacaactgctgatgaattttgcttttactatgcaagaaaagatgctgggttat
gctttccagacttcccgagaaagcaagtcagaggaccagcatctaactacttgggccagatggaagactacgagaaagtgggggggatcagcagaanaca
caaacacaactgcctctgtgtccangaagtcatngagtgggctgcggcagcctgtgagcgctgtgcacagcgggggatgggctccatcgggctcttcatt
ctagagaaagaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: