XB-CLONE-4351404
Clone Name: IMAGE:7026996
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Gene:
krt70
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784914
[+]
taattaatcccccccccccccgatcagctcatacaggggaacacatcactgctacattgggccccttaacattacagcatcacaatgagtttctctaccc
gaagtgtctctcaatcctcccgctatggtgttctggcatcacccatggtgaatcgggccctaagtgtggcagggggtgcccagactgtccggatgtcctc
tgctaaactgaccagctctgcgtttgccggaccctctgcccttgatgttagctcctttggcaacaatggcaaggagacaatgcagaacctgaatgatcgt
ctggccagctacctggaacgggtccgttccttggagcaagccaaccatgagctggagctgaagatcagggagtactgtgagaagaaagctgccattggct
cccctgatgttagcggttactataacatcatcaacttactgcgctcccagatcaatgatgcaagcattgacaatgccaggctggtcctccagatcgacaa
tgccaagctggcagctgatgacttcaaaatcaagtatgagtcagagctggccattaggactggagtcgaaagcgacattatcggactgcgcagagtcctc
gatgagctgaccctcaataagacagacctggagttggagattgaaagtctgaaaggaagagctgattttacctaaaggaaaaatcatgaaagaggaaact
ttgctggttgcctaggggcccagttgccaagaaggcccaaagtttggatggtaacaggggttgaatttctggccttccctcccttggttgaacccttgtt
caacaaaaattacatgggttcctggaattggttgaaagaggggcc
3' EST: CF784913
[+]tttttttttttttttaacttagaattctttatttggatgcattgtccctgaggaagagttaaacacaaacagcctaggtttgcattacatgacatttatt
ttgctaatttctcttctgctttcatatcttctctgtcatattaaacatttacgtataggctacattttaaaatgaagcttcttggttgccttcaagacta
tgttatttcaactcttctgctgccctttctcaggtacaggctcttaattcttaagattctttttaggatttctcagtcacttcctcgaccctagaagaga
ccactttcccgtcgactacttcttcgatcaccgtcttcacctttttgatggagctcttgctttgttccttactgacgatggtaacagctttctgtgtttc
agtctgggaaaatctggtgtcttctccttctagcaggcgtctgtaggtggaaatttccatttccagccttgtcttggcatcgagcaacaacttatactcc
aaagactggcgctcggcatctgaccgtaagttttgaaggtctaattctatttgcgtgaccatggcctgaaggtggttgagctcagctccatatcgggcct
cggtttctgccaaagtgccttccagtgctcctttcatgctcaagagtgactgaagttcaatctccaggctctgtattgtgcgcttcaggtctgtaatctg
cgacttaaatgtctggagagcagctgtgttggtggcaacttnctttgttgagattttcgctctgtcctctgtaccaagcttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: