XB-CLONE-4351727
Clone Name: IMAGE:7027127
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Gene:
azin2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784709
[+]
taattaatccccccccccccgagcgcatacactgtacacagagctggtgcggtttctaggcttagttccacatttacattaatgggcaacggccgtgtca
atgcatgttaagaaagcgacagacgccagcagctgcgactacatccacagcctggggtgctgcaaaatctccgagtttactgctgagcccccctggttcc
tttgataaaagcttgttgcctgcttaaaaactatcttgcgtaaaaggcttaaaaccctagatttcacaatgcaaggataaatccgggagtcagattttat
tttggtttatgaaggctttatggccctagatttgacggaagaagatcactaatgaagtctcacagacttggcgatcggtaaccgccttttttcgggggtg
gatcgtggggggctgccgctaaacaaatcccctcccaccttcctttaaaggattttgccttctttgtcgaaaccattaccccataaccccgttacttgtc
cactttgttacctccgtgcacccaccatcaccttctttgttcctcaaccttcgtattgtttaccatcttcacaatgtcgtcctgctcatccccgatcttt
ttctccactttccgttcccttcgataaatatattgcgtgtgtgcaactcaccgtttttacccccacttcgatttgggggtgcccgaagacaggtaaaaac
aataatgtcgtaacttatgtcgccattattacttcacaacaatttgtcctttgtttattaagaaccgagagttcgtaataaatacatagtctatacaaat
attttttctcccctatttttcccacccacccaacattgtttataccacaagagtgcctggaaactaatataaaccaaacgcccgtataaagtcccttctg
atccccccaacatattttccccacactcgtttttttatacaaacccgatttttcccgacaaaataacactctctctacacaacgttttatcgtgttcagc
cactggggtaaataaacggtgggcccacaactgatatatatcaatatatttttttcccacacacacaccctcgtagaaattttttaactaaagcacccgt
aatccg
3' EST: CF784708
[+]tttttttttttttttcttttttttttttttttttttttttttttttttttttaaaanaaatagaatttattataaatgaataagatgaactattttcagt
gcataaactgttccagcatggatgatccaaaaacaaaatactgtttgttgcatacttaaaaacaatgagataaataaggattggtccaaaaataaatatc
aatatgtgtttctcccatgacacaaaaatttggatgatgatggctgcatgcaaaggtaaaaatgattcctttaacaatacaatgaactacttagatgagc
taatactatcccattgtttaatggcaaaacatttgcatgtcactcccatggtgcaggaattcaaataatgctggtggctgcaaaggtacgtgtgaagcac
aaggaatccaaaatctcccagccacaaaacattggggtgcaaacagtttccttatccccagttccctgtaatcccctctgcaacaactcaactgctttcc
aagcagcacggggcatggcataatgtactggaaattgctggaaaccgttaaaattaaaaaatgccgctatggtgtatgcacccatgtttttcaaacaaaa
accagttctccaaaatgatgcctcaggggagccaaaaggttcccttgcatttgatccaagccctcccatgtagggcccccaaaggcctactgggtgtaaa
agggccgaatctggaaaaggttttcttatggaggaatccggtttaaggggtgaccttggattcaaagacccaacccattttaagggggatcccctatccc
ccccccctttaaaccttatcaccttgagggtctct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: