XB-CLONE-4352065
Clone Name: IMAGE:7027259
|
Gene:
eef1a1o
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784490
[+]
taattaatccccccccccccccgctcgcagtctctgcctgctctgctgtatttgttcgtgggggttgactggtgttcgctgccaagatgggaaaggaaaa
gattcacattaacatcgttgtgatcgggcatgtggactctggcaagtcaaccacaaccgggcatctcatctacaaatgcgggggcattgacaaaaggacc
atcgaaaagtttgagaaggaagctgcagagatgggtaaaggctcctttaaatatgcttgggtcttggataagctgaaagccgagagagaacgtggcataa
ccattgatatttccttgtggaagttcgagactggaaaattctatatcacaattattgatgctcctgggcacagagacttcatcaaaaacatgatcactgg
cacttctcaggctgactgtgcagtgctcattgttgctggtggtgttggagaatttgaggctggtatctctaaaaatggacagactcgagagcatgccctc
ttagctttcactctaggtgtcaagcagcttataattggagtaaacaagatggactccactgagccccctttcagccaaaaaagatttgaggaaattacta
aagaagtcagtgcctactttaagaagattggctaccacccagcgactgttccatttgtgccgatatctggatggcatggagacaacatgctgggggctag
caccaatatgcccctgggtttaaaagggtggaaagattggaaagggaaagaagggaaattgccggtgggtgtggacaccgtgcctagaaaccaccttgga
tttgcaatttaattccccccccttaagcca
3' EST: CF784489
[+]tttttttttttttttcatcattaaacatttattcatttttacaggacaccaagattacactgtacagggttacacagatcatttcttgccagcttttaca
gcagacttagtcacttttccagagcttgctgcctttttatccacacctttgattacgccaacagctaccgtctgcctcatgtcacgcactgcaaatcggc
caaggggtgggtagtctgagaaggtttctacacacatgggcttcccaggaatcatttccactatagctgcatctccagatttcaagaattttgggtcatc
ctccagcttcttgccacttcttctgtctatcttttgcttcagctcagcaaacttacaggcaatatgagctgtatggcagtccaggactggagcatagcca
gcactaatctgtcctgggtgattgagtataattacctgtgctgtaaaggttccagcttgcataggtggatcatttttgctatcaccagccacattgcctc
tcctgatgtccttcacagatatattctttacattaaagcccacattgtctccaggcaatgcctcttgcaaggcctcatgatgcatttccacagacttaac
ttctgtagtaacattactaggtgcaaaagtcacaaccatgccaggcttcaagacaccagtctccactctgccaacaggcacagtgccaattccacccatc
ttgtacacatcctgcagaggggagccttanaggcttgtcagtggggtcgctgaangaggaataatgcaaatcagtgctttctagcagtgtcaccaccact
ggcattttnccttctttccttttcaatctttccagcn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: